脑出血后神经细胞凋亡的时相变化及果糖二磷酸钠镁对其的抑制作用  

The time course of neuronal apoptosis induced by intracerebral hemorrhage and the inhibitory effect of sodium magnesium fructose diphosphate

作　　者：曾凡新[1] 陈有香[2] 董志[2] 

机构地区：[1]重庆医药工业研究院药理毒理学室,重庆400061 [2]重庆医科大学药理学教研室,重庆400016

出　　处：《中国药理学与毒理学杂志》2003年第4期289-291,共3页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的　研究脑出血 (ICH)后细胞凋亡的规律及果糖二磷酸钠镁 (SMFD)的作用。方法　尾状核注射胶原酶诱导大鼠ICH模型 ,用TUNEL染色法观察ICH后神经细胞凋亡的发生及其随时间变化的规律以及SMFD的作用。结果　ICH触发了神经细胞凋亡 ,造模后 6h即出现凋亡细胞 ,高峰时间在出血后 2 4～ 72h ,7d时仍有凋亡细胞存在。凋亡细胞主要分布在海马及大脑皮质 ;SMFD可明显减少ICH后神经细胞凋亡数目 ,其作用强于相似剂量的 1,6 二磷酸果糖或硫酸镁。结论　ICH可触发神经细胞凋亡 ,2 4～ 72h细胞凋亡数目达高峰 ;SMFD对ICH后神经细胞凋亡有显著的抑制作用。AIM To study neuronal apoptosis and its time course of rat brain subjected to intra cerebral hemorrhage(ICH), as well as the inhibitory effect of sodium magnesium fructose diphosphate(SMFD) on it. METHODS ICH was induced in adult rats by stereotaxical injection of bacterial collagenase into left caudate nucleus, the time course of neuronal apoptosis and the effects of SMFD on it were determined by counting the number of positive cells after stained with TUNEL method. RESULTS ICH triggered neuronal apoptosis, which occurred at 6 h after the onset of ICH, peaked at 24-72 h, then descented and lasted for 7 d. Apoptotic cells mostly distributed in hippocampus and cortex; SMFD decreased the number of apoptotic cells and its effect was more potent than that of similar dose of 1,6 diphosphate fructose or magnesium sulfate. CONCLUSION ICH triggered neuronal apoptosis, the peak of it was at 24-72 h after the onset of ICH, and SMFD significantly inhibited neuronal apoptosis.

关 键 词：脑出血 凋亡 果糖二磷酸类 硫酸镁 

分 类 号：R743.34[医药卫生—神经病学与精神病学]