两个不同产地大蒜挥发油成分的比较研究  

Comparative study of volatile oil components in garlic from two different habitats

作　　者：曾仲大[1] 梁逸曾[1] 李博岩[1] 胡芸[1] 

机构地区：[1]中南大学化学化工学院中药现代化研究中心,湖南长沙410083

出　　处：《中草药》2003年第9期772-776,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金重点项目 ( 2 0 2 350 2 0 2 0 1 750 36 )

摘　　要：目的　利用色谱联用技术 (GC-MS)对陕西兴平基地大蒜和湖南普通食用大蒜中的挥发油成分进行测定 ,比较挥发油成分的异同。方法　采用质谱相关色谱理论 ,比较了这两个不同地区大蒜挥发油成分 ;同时基于直观推导式演进特征投影法 (HEL P)对产生的二维气相色谱 /质谱数据进行解析 ,根据得到的色谱保留时间和纯质谱在质谱库中进行相似性检索 ,实现对组份的鉴定。结果　鉴定并用总体积积分法定量计算了 19个挥发油成分。质谱相关色谱理论和 HEL P方法得到了相同的定性信息。结论　采用化学计量学的方法 ,成功地实现了两个地区大蒜挥发油成分的比较研究 ,得到了两者整体组份信息的异同 ,为药理药效的比较研究奠定了基础。Object To detect and compare chemical components of volatile oil in common edible garlic from Xingping base, Shaanxi Province and H unan Province Methods By the theory of mass spectrum correlation chromatog raphy, their similarities and differences of the volatile oil were compared Th en , heuristic evolving latent projections (HELP) resolution and overall volume int egration method upon two-dimensional GC-MS data were employed to determine and quantify the components Results The qualitative informati on was found by the above theory and HELP, and 19 volatile oils were identified and caculated for the common edible garlic from Xingping base, Shaanxi Province and Hunan Province Among them, Seven components were not reported in the known referen c es before Conclusion The resolution results by HELP are coi ncident with the results of mass spectral correlation chromatography Their qua n titative information has great differences of the two different places' garlic All these results provide powerful evidence for future pharmacological research

关 键 词：大蒜 挥发油 气相色谱-质谱联用 质谱相关色谱 直观推导式演进特征投影法 

分 类 号：R284.1[医药卫生—中药学]