抗膜抗原的单抗对痢疾杆菌入侵HeLa细胞的影响  

Influences of McAb against S.flexneri-2a on HeLa cell invasion

作　　者：王岚[1] 高杰英[1] 彭虹[1] 王华[1] 易绍琼[1] 

机构地区：[1]军事医学科学院微生物流行病研究所免疫室,北京100071

出　　处：《中华微生物学和免疫学杂志》2003年第8期633-635,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的　观察抗痢疾杆菌膜蛋白和LPS的单抗对痢疾菌入侵HeLa细胞的影响。方法　采用不同膜抗原的单抗处理痢疾杆菌后进行HeLa细胞入侵及阻断实验 ,观察不同膜抗原单抗对痢疾杆菌入侵的作用。结果　观察到抗菌膜蛋白IpaB的单抗和LPS的单抗均不能阻断细菌的入侵且能促进细菌的入侵 ,在HeLa细胞胞浆内出现成簇的S .flexneri 2a菌 ,入侵菌数远远超过未经单抗处理的S .flexneri 2a菌感染的HeLa细胞组。结论　提示痢疾杆菌的抗菌膜蛋白单抗和LPS单抗所识别的表位 ,均具有调节S .flexneri 2a菌进入HeLa细胞的能力。Objective To identify the effects of McAbs against membrane protein or LPS of S.flexneri 2a on invading HeLa cells by Shigella flexneri . Methods After treated with McAbs against various membrane antigens, Shigella invaded the HeLa cells. The invasion results were analyzed by microscopy and laser scanning confocal microscopy. Results McAb 17G2, 3A6, 1G8 and 2A3 failed to block the course of invasion while increased the number of S.flexneri 2a in HeLa cells. Conclusion The epitopes identified by these McAbs are associated with the invasion process, which may be a component of protein secretion system.

关 键 词：痢疾杆菌 HELA细胞 入侵 抗膜抗原 单克隆抗体 

分 类 号：R562.25[医药卫生—呼吸系统] R-332[医药卫生—内科学]