糖尿病视网膜病变中血管内皮细胞生长因子与血管病变的关系  

Relationship between Vascular Endothelial Growth Factor and Lesion of Blood Vessel in Diabetic Rat

作　　者：宋鄂[1] 董宇[1] 赵秀珍[1] 徐琪[2] 王心蕊[3] 吴家祥[3] 

机构地区：[1]吉林大学第一医院眼科,吉林长春130021 [2]吉林大学基础医学院病理生理学教研室 [3]吉林大学基础医学院病理解剖学教研室

出　　处：《吉林大学学报（医学版）》2003年第5期624-626,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题 (990 5 75 - 5 ) ;吉林大学新技术新疗法课题 (2 0 0 0 10 9)

摘　　要：目的 :研究糖尿病大鼠视网膜病变 ( DR)时血管内皮细胞生长因子 ( VEGF)与血管器质性改变的关系。方法 :建立糖尿病大鼠模型 ,随机分为正常对照组 ( M)及糖尿病 1月组 ( M1)、3月组( M3 )、5月组 ( M5)。分别在视网膜血管铺片上行 VEGF原位杂交、免疫组化 ,并分别对视网膜血管进行透射电镜观察。结果 :1周细胞数量 M1组与 M组相比无差别 ( P>0 .0 5 ) ,M3 及 M5组与 M组相比明显减少 (分别 P<0 .0 1 ,P<0 .0 0 1 ) ;2毛细血管形态以 M5组病变最重 ,可见毛细血管栓塞 ,无细胞毛细血管 ;3VEGF原位杂交仅 M5组表达为 34% ;4VEGF免疫组化仅 M5组有表达 ,为 5 6% ;5血管的透射电镜观察 M组未见异常改变 ,从 M1组开始出现异常改变 ,以 M5组最明显。表现为基底膜节段性增厚、断裂缺失 ,内皮细胞肿胀变形 ,向管腔内指状突起 ,异染色质浓集居边 ,周细胞核异染色质浓集居边 ,线粒体肿胀变性 ,甚至呈空泡状。结论 :DR时血管器质性改变在先 ,而生长因子表达在后。Objective:To study the relationship between vascular endothelial growth factor and lesion of blood vessel in diabetic rat. Methods: Diabetic rat model which were classified normal as control, diabetic for one month (M 1), three month(M 3), five months (M 5) were set up. In situ hybridation and immunohistochemistry were done on retinal digest preparation, then retinal vascular was obsreved by transparent eletron microscope. Results:① Number of pericytes,there is no difference of the number of pericytes between M 1 and M ( P >0.05), but the number of pericytes decreased obviously in M 3 and M 5 (respectively P <0.01, P <0.001). ②The morphology of capillary,the most serious change is in M 5, including thrombus of capillary and lack of capillary. ③VEGF situ hybridation,the only expression in M 5 was 34%. ④VEGF immunohistochemistry,there was expression in M 5 and the expression was 56%. ⑤Observation of vascular by transparent electron microscope, there was no change in M. The change begins from M 1 and becomes serious in M 5. It showed as follows, fragmental thicker or split of basement membrane; Swell of endothelium cell and finger like process to the capillary cavity; Disconcentration of heterochromatin; Disorder of the ultrastructure discus membrane of external fragment and swell of mitochondrin of internal fragment of optic cell. Conclusions: The change of the morphology of vascular is first in diabetic retinopathy, the expression of growth factor follows. [

关 键 词：糖尿病视网膜病变 血管内皮细胞生长因子 原位杂交 免疫组化 超微结构 视网膜血管铺片 

分 类 号：R587.2[医药卫生—内分泌] R774.13[医药卫生—内科学]