双标记原位杂交法检测间期细胞BCR/ABL融合基因  

Detection of BCR/ABL Fusion Gene in Interphase Cells with Bi-labelling Hybridization in Situ

作　　者：袁长吉[1] 王建伟[2] 李舜华[1] 易永林[1] 

机构地区：[1]吉林大学第一医院血液肿瘤科,吉林长春130021 [2]吉林大学基础医学院病理解剖学教研室

出　　处：《吉林大学学报（医学版）》2003年第5期637-639,共3页Journal of Jilin University：Medicine Edition

摘　　要：目的 :应用双标记原位杂交方法检测 BCR/ ABL融合基因。方法 :BCR基因探针用地高辛标记 ,碱性磷酸酶显色 ;ABL基因用3 H- d ATP标记 ,核子乳胶放射自显影。结果 :检测 9例初治的慢性粒细胞白血病 ( CML)病人均为阳性 ,阳性细胞比例为 93% ;检测 CML来源的 K5 62细胞株阳性细胞占 98.8% ;正常人假阳性率为 0 .75 %。结论 :该方法简便、快速 ,适用于Objective:To Detect BCR/ABL fusion gene with bi labelling hybridization in situ. Methods:BCR gene probe was labeled by cardiox, showed color with alkaline phosphatase. ABL gene were labeled by 3H Datp, radiating self developing with nucleon lacteprene. Results:BCR/ABL fusion gene all were positive in 9 chronic myelocytic leukemia(CML) patients in initial treatment, the ratio of positive cells was 93%, and the ratio of positive cells was 98.8% in K 562 cell strain source from CML. It showed that the results accord with objective changes. Pseudo positive rate was 0.75% in normal persons. Conclusion:The methods is both easy and fast,and it is suitable to detect minimal residual disease of chronic myelocytic leukemia (CML). [

关 键 词：慢性粒细胞白血病 原位杂交 BCR/ABL融合基因 基因探针 地高辛 

分 类 号：R733.72[医药卫生—肿瘤] R730.4[医药卫生—临床医学]