基质降解酶u-PA、PAI-1与人口腔鳞状细胞癌细胞侵袭转移的相关性研究  

The correlation between the expression of matrix degrading enzymes u-PA,PAI-1 and invasion and metastasis of human oral squamous cell carcinoma

作　　者：金晓明[1] 李雅馨 李金荣[3] 李宁毅[1] 贾暮云[1] 

机构地区：[1]青岛大学医学院附属医院,266003 [2]青岛市口腔医院 [3]武汉大学口腔医学院

出　　处：《现代口腔医学杂志》2003年第5期398-400,共3页Journal of Modern Stomatology

基　　金：青岛大学医学院科研启动基金资助 (编号 :博 2 0 0 1-0 3 )

摘　　要：目的　探讨体外培养细胞系TSCCa和GNM细胞的u -PA及PAI - 1活性和表达与口腔鳞状细胞癌侵袭转移的关系。方法　采用酶联免疫反应法和免疫组化法测定了两细胞系中u -PA及PAI - 1的活性和表达 ,同时对GNM细胞在BALB/CA裸鼠皮下种植瘤组织的u -PA及PAI - 1抗原表达进行了测定。结果　两细胞系均能产生u -PA及PAI- 1,但u -PA在两细胞系中的表达不同 ,在GNM细胞u -PA为高表达 ,在TSCCa细胞中u -PA为低表达 ,PAI- 1在两细胞系中均为高表达 ;种植瘤组织中u -PA呈阳性～强阳性表达 ,PAI- 1为阴性表达。结论　uObjective To detect the relationship between the activities and expression of u-PA,PAI-1 and its association with invasion and metastasis of human oral squamous cell carcinoma in vitro.Methods The activities and expression of u-PA,PAI-1 were determined by ELISA and immunohistochemistry in TSCCa and GNM cell lines;Immunohistochemically methods were used to analyze the expression of u-PA,PAI-1 in GNM cells implanted in nude mice subcutaneously.Results Both TSCCa and GNM had a high level of u-PA and PAI-1;while u-PA expression was different between two cell lines;u-PA had a high level in GNM cells,but a low level in TSCCa cells,PAI-1 had a high level of expression in two cell lines;that u-PA had a high level of expression in vivo,PAI-1 had a low level of expression.Conclusion Matrix degrading enzymes of u-PA may play an important role in invasion and metastasis of oral squamous cell carcinoma.

关 键 词：口腔鳞状细胞癌 基质降解酶 U-PA PAI-1 酶联免疫反应法 免疫组化法 

分 类 号：R739.8[医药卫生—肿瘤]