幽门螺杆菌长期感染蒙古沙土鼠导致胃炎、胃溃疡的实验研究  

Establishing Mongolian gerbils models of gastritis and peptic ulcer by long-term infection with Helicobacter pylori

作　　者：李勣[1] 刘纯杰[1] 陶好霞[1] 刘秀丽[1] 李淑琴[1] 张兆山[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《军事医学科学院院刊》2003年第4期255-258,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家"8 63"高技术基金资助项目 ( 2 0 0 1AA2 15 161)

摘　　要：目的 :用幽门螺杆菌 (Helicobacterpylori)长期感染蒙古沙土鼠建立胃炎、胃溃疡的动物模型。方法 :蒙古沙土鼠经过禁食、禁水预处理之后 ,采用国际标准菌株H .pyloriATCC 4 35 0 4灌胃 ,在末次灌胃动物后 4 ,8,12 ,2 4 ,4 8,5 2周分别对H .pylori感染组和正常对照组蒙古沙土鼠的胃黏膜进行H .pylori形态学 (涂片和细菌培养 )、H .pylori快速尿素酶检测、H .pylori特异性PCR检测和病理组织学观察 ,并对血清中抗H .pyloriIgG抗体进行了检测。结果 :H .pylori感染组动物以上各项指标均为阳性 ,表明已经感染H .pylori;而正常对照组则以上各项均为阴性。病理组织学观察表明感染组 8周即可见到慢性炎症性病变 ,12周时黏膜层和黏膜下层可见到淋巴滤泡 ,2 4周胃窦部可见到胃溃疡。结论 :H .pyloriATCC 4 35 0 4可以长期定植于蒙古沙土鼠腺胃 ,能够产生与H .Objective:To develop a Mongolian gerbil model mimicking human gastritis and peptic ulcer disease by long_term infection with Helicobacter pylori .Methods:Each Mongolian gerbil was inoculated with H.pylori ATCC 43504 after subjected to a 24_hour fasting period. Noninfected control gerbils and infected gerbils were sacrificed at 4,8,12,24,48,and 52 weeks after H.pylori infection. A piece of gastric mucosa obtained from the posterior wall of the antrum of each gerbil was used for culturing of H.pylori , rapid urease testing, PCR of the special genes and histopathological examination. Serum was obtained to measure the IgG antibody level to H.pylori .Results:At 8 week after inoculation, chronic inflammation was observed in the pyloric mucosa by histopathological examination. At 12 week after inoculation, lymphoid follicles were especially conspicuous in the submucosa, and they were also found in the deep portion of the mucosa. At 24 week after inoculation, the gastric ulcer was observed in the infected Mongolian gerbils.Conclusion: H.pylori ATCC 43504 can colonize easily in the glandular stomach mucosa of Mongolian gerbils, and the histopathological changes were similar to those of human with H.pylori infection. [

关 键 词：幽门螺杆菌 蒙古沙土鼠 动物模型 胃炎 胃溃疡 

分 类 号：R573[医药卫生—消化系统]