树突细胞对肿瘤浸润淋巴细胞抗乳腺癌免疫活性影响的研究  

Study of Anti Breast Cancer Activity of Tumor Infiltrating Lymphocytes Affected by Dendritic Cells

作　　者：刘剑勇[1] 张春燕[2] 赵荫农[1] 唐凯[2] 张力图[2] 李挺[1] 

机构地区：[1]广西医科大学附属肿瘤医院肝胆乳腺外科,广西南宁530021 [2]广西医科大学附属肿瘤医院细胞与分子生物学研究室,广西南宁530021

出　　处：《癌症》2003年第10期1030-1033,共4页Chinese Journal of Cancer

基　　金：广西科学研究与技术开发计划攻关项目(桂科攻No.0143052)

摘　　要：背景与目的:树突细胞(dendriticcell,DC)又称树突状细胞,是目前已知的功能最强的抗原呈递细胞,它可以在体内、外向T淋巴细胞呈递抗原,并诱发细胞毒T淋巴细胞(cytotoxicTlymphocyte,CTL)反应。本研究旨在探讨DC激活的肿瘤浸润淋巴细胞(tumorinfiltratinglymphocytes,TIL)体外对乳腺癌细胞的杀伤活性。方法:从乳腺癌患者外周血获取DC,应用粒/巨噬细胞集落刺激因子(granulocyte/macrophagecolonystimulatingfactor,GM-CSF)、白细胞介素-4(interleukin-4,IL-4)和肿瘤抗原激活DC,然后用DC激活TIL,观察TIL在体外对自体乳腺癌细胞和Bcap-37乳腺癌细胞的杀伤活性。结果:DC激活的TIL对自体乳腺癌细胞具有很强的杀伤活性,杀伤率为(85.76±2.93)%,明显高于未经DC激活的TIL、DC激活的T淋巴细胞和未经DC激活的T淋巴细胞对自体乳腺癌细胞的杀伤率犤分别为(52.11±1.48)%、(51.35±1.46)%和(3.59±0.25)%犦。而它们对Bcap-37乳腺癌细胞的杀伤活性则相对较低犤分别为(40.03±1.29)%、(22.09±0.87)%、(21.66±0.85)%和(1.76±0.14)%犦。结论:乳腺癌患者外周血DC能诱导TIL产生高效而特异的抗乳腺癌免疫活性。BACKGROUND &OBJECTIVE: Dendritic cell (DC) is the strongest antigen presenting cell(APC). It can present antigen to T lymphocytes in vivo and in vitro,and induce cytotoxic T lymphocyte(CTL) reactions.This study was designed to investigate the killing activity of tumor infiltrating lymphocytes (TILs) stimulated by dendritic cells on breast cancer cells in vitro. METHODS: DCs were isolated from peripheral blood of patients with breast cancer. DCs were stimulated by granulocyte/macrophage colony stimulating factor(GM CSF), interleukin 4(IL 4), and tumor antigen. Then TILs were stimulated by DCs and their killing activity on autogenous breast cancer cells and Bcap 37 breast cancer cells in vitro were observed. RESULTS: TILs stimulated by DCs had very high killing activity on autogenous breast cancer cells and the killing rate was (85.76±2.93)%. The killing rate was higher obviously than that of TILs not stimulated by DCs and T lymphocytes stimulated by DCs or not on autogenous breast cancer cells,respectively [killing rates:(52.11±1.48)%,(51.35±1.46)%, and (3.59±0.25)%, respectively]. However, their killing activities on Bcap 37 breast cancer cells were lower [killing rates:(40.03±1.29)%,(22.09±0.87)%,(21.66±0.85)%,and (1.76±0.14)%,respectively]. CONCLUSION: The results indicate that DC from the patients with breast cancer can induce TIL to produce efficient and specific anti breast cancer immune response.

关 键 词：树突细胞 肿瘤浸润 淋巴细胞 乳腺癌 免疫活性 

分 类 号：R737.9[医药卫生—肿瘤] R329.2[医药卫生—临床医学]