反义LeEIL2基因表达载体的构建及在番茄中的转化  

Construction of Plant Antisense Gene Expression Vector pBI121-LeEIL2 and Transformation in Tomato

作　　者：薛印革[1] 朱本忠[1] 薛萍[1] 田慧琴[1] 罗云波[1] 

机构地区：[1]中国农业大学食品学院,北京100083

出　　处：《河南农业科学》2003年第10期47-50,共4页Journal of Henan Agricultural Sciences

摘　　要：为了研究LeEIL2基因在番茄果实成熟过程中的作用 ,将全长的番茄LeEIL2基因以反义方向构建到植物表达载体 pBI1 2 1上 ,经过酶切鉴定正确后 ,将该载体转入农杆菌EHA1 0 5中 ,通过农杆菌介导法转化番茄 ,经组织培养和抗性筛选获得再生植株 ,PCR检测再生植株 ,初步鉴定为阳性植株 ,为获得转反义LeEIL2番茄果实打下基础。In order to investigate the role of LeEIL2 in tomato fruit ripening,the full-length LeEIL2 gene was inserted in reverse orientation into expression vector pBI121,and subsequently confirmed with restriction enzyme analysis. The antisense expression vector pBI121-LeEIL2 was mobilized into Agrobacterium tumefacious EHA105 by chemical-based DNA direct transformation. Transgenic tomato was developed using Agrobacterium-mediated transformation with kanamycin resistance selection. The presence of antisense LeEIL2 in transformants were confirmed by PCR and transgenic tomato fruit will be obtained later.

关 键 词：LeEIL2基因 番茄 反义表达载体 遗传转化 农杆菌介导 乙烯 EIN3基因 

分 类 号：S641.2[农业科学—蔬菜学]