马铃薯A病毒CP基因的克隆与序列分析  

The clone and sequence analysis on the CP gene of potato virus A

作　　者：吴兴泉[1] 陈士华[1] 吴祖建[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所,福州350002

出　　处：《植物保护》2003年第5期25-28,共4页Plant Protection

基　　金：福建省农业厅和国家教育部资助项目 (0 0 183 )

摘　　要：利用根据马铃薯A病毒 (PVA)外壳蛋白 (CP)基因序列设计合成的一对引物 ,以带毒植物总RNA为模板 ,RT-PCR扩增得到长 0 8kb的目的片段。将目的片段转入大肠杆菌并进行了序列测定。测序结果与PVA其他分离物CP基因序列比较 ,发现其核苷酸同源性最高可达 99%。With the specific primers which were designed based on the potato virus A (PVA) coat protein (CP) gene sequence, one gene fragment (0.8 kb) was amplified by reverse transcription polymerasae chain reaction (RT PCR) using the total RNA of the plant infected by PVA. The gene was cloned into Escherichia coli DH5α and sequenced. The sequence was compared with the sequence of the homologous gene of other isolates of PVA The result showed it had high homology with the other isolates (the highest homology could reach 99% of nucleic acid). Based on CP amino acid sequence the phylogenic tree of PVS was established and the isolates were clustered into many groups.

关 键 词：马铃薯 病毒 基因 克隆 蛋白 蚜虫 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]