Improvement of Properties of Pseudomonas sp.Lipase in Resolution of 2-Octanol  

作　　者：WANGZhi QUANJing WENGLiang ZHENGLiang-yu LIUNing DONGHuan CAOShu-gui 

机构地区：[1]KeyLaboratoryforMolecularEnzymologyandEngineeringofEducationalMinistry [2]CollegeofLifeScience,JilinUniversity,Changchun130023,P.R.China

出　　处：《Chemical Research in Chinese Universities》2003年第4期446-449,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China( No.2 0 0 72 0 11and 2 0 2 72 0 17)

摘　　要：A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na 4P 2O 7·H 2O and Na 4P 2O 7 can dramatically activate the modified enzyme.A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na 4P 2O 7·H 2O and Na 4P 2O 7 can dramatically activate the modified enzyme.

关 键 词：LIPASE SURFACTANT Salt hydrate pair ESTERIFICATION ENANTIOSELECTIVITY 

分 类 号：Q556[生物学—生物化学]