缺血性脑损伤基质金属蛋白酶-9的表达与梗死面积研究  

The change of matrix metalloproteinase-9 early expression after cerebral ischemia-reperfusion injury

作　　者：刘华[1] 廖维靖[1] 杨万同[1] 郑婵娟[1] 

机构地区：[1]武汉大学中南医院康复医学科,湖北武汉市430071

出　　处：《中国康复理论与实践》2003年第10期600-601,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目 (39970 935和 30 2 71 671 )

摘　　要：目的观察大鼠脑缺血再灌后基质金属蛋白酶 9(MMP 9)的表达情况 ,探讨该酶在短暂性局灶脑缺血损伤中的作用。方法线栓法制作大鼠脑缺血 /再灌注不同时点模型 ,采用免疫组化法观察MMP 9的表达 ,同时测定梗死面积。结果MMP 9主要在缺血神经元、血管内皮细胞、嗜中性粒细胞中表达 ( 6h开始表达 ,2 4— 48h表达最强 ,P <0 .0 1)。结论脑缺血再灌后诱导MMP 9表达增加 ,且与脑梗塞面积紧密相关。ObjectiveTo explore the relationship of the expression of matrix metalloproteinase-9 (MMP-9) and the infarction area after transient focal cerebral ischemia and reperfusion.MethodsModels of transient focal ischemia of MCA in rats were built by placing an intraluminal suture and the neurologic deficit score, lesion area and MMP-9 expression were analyzed.ResultsThe expression of MMP-9 and the infarction area were positive correlation, but there is no correlation between the neurologic deficit score and the MMP-9 expression.ConclusionAfter cerebral ischemia-reperfusion the MMP-9 expression is upregulated. The study suggests that MMP-9 may be involved in infarction lesion.

关 键 词：基质金属蛋白酶-9 缺血性脑损伤 梗死面积 表达 

分 类 号：R743.3[医药卫生—神经病学与精神病学]