Down-regulation of γ-synuclein in human esophageal squamous cell carcinoma  

作　　者：Cui-QiZhou ShuangLiu Li-YanXue Yi-HuaWang Hong-XiaZhu NingLu Ning-ZhiXu 

机构地区：[1]LaboratoryofCellandMolecularBiology,CancerInstituteandCancerHospital,ChineseAcademyofMedicalSciences,PekingUnionMedicalCollege,Beiiing100021.China [2]DepartmentofPathology,CancerInstituteandCancerHospital,ChineseAcademyofMedicalSciences,PekingUnionMedicalCollege,Beijing100021,China

出　　处：《World Journal of Gastroenterology》2003年第9期1900-1903,共4页世界胃肠病学杂志（英文版）

基　　金：the National Natural Science Foundation of China, No.39925020;State Key Basic Research Program,No.G1998051204

摘　　要：AIM: To investigate gene expression pattern of human γsynuclein gene in human esophageal squamous cell carcinoma (ESCC) by using semi-quantitive reverse transcription polymerase chain reaction (RT-PCR), and to study the role of γ-synudein in the development of human ESCC.METHODS: Semi-quantitive RT-PCR of 27 pairs of specimens of human ESCC tissues and corresponding normal tissues was used to investigate the expression pattern of γsynuclein in ESCC. 9706/γ-syn cells in which γ-synuclein was overexpressed were obtained through cloning γ-synuclein gene by PCR and transfecting it into ESCC 9706 cells, then selecting with G-418 for 14 days. The biological effects of γsynuclein were measured and compared between 9706/γ-syn and 9706/vec cells by cell growth curve and soft agar assay.RESULTS: RT-PCR showed that γ-synuclein gene was expressed in all the 27 cases of normal epithelial tissues,while downregulation of γ-synudein was observed in 16 out of the 27 cases (59.3%) of ESCC. There were also 6 cases of ESCC tissues with a high expression level of γ-synuclein mRNA. In functional analysis we found that over-expression of γ-synuclein in ESCC 9706 cells could inhibit the growth rate and transformation ability of ESCC 9706 cells.CONCLUSION: The low expression level of γ-synuclein in human ESCC and the biological effects of γ-synuclein overexpression on ESCC 9706 cells suggest that γ-synuclein may play a role as a negative regulator in the development of human ESCC.AIM:To investigate gene expression pattern of human γ- synuclein gene in human esophageal squamous cell carcinoma(ESCC)by using semi-quantitive reverse transcription polymerase chain reaction(RT-PCR),and to study the role of γ-synuclein in the development of human ESCC. METHODS:Semi-quantitive RT-PCR of 27 pairs of specimens of human ESCC tissues and corresponding normal tissues was used to investigate the expression pattern of γ- synuclein in ESCC.9706/γ-syn cells in which γ-synuclein was overexpressed were obtained through cloning γ-synuclein gene by PCR and transfecting it into ESCC 9706 cells,then selecting with G-418 for 14 days.The biological effects of y- synuclein were measured and compared between 9706/γ-syn and 9706/vec cells by cell growth curve and soft agar assay. RESULTS:RT-PCR showed that γ-synuclein gene was expressed in all the 27 cases of normal epithelial tissues, while downregulation of γ-synuclein was observed in 16 out of the 27 cases(59.3 %)of ESCC.There were also 6 cases of ESCC tissues with a high expression level of γ-synuclein mRNA.In functional analysis we found that over-expression of γ-synuclein in ESCC 9706 cells could inhibit the growth rate and transformation ability of ESCC 9706 cells. CONCLUSION:The low expression level of γ-synuclein in human ESCC and the biological effects of γ-synuclein over- expression on ESCC 9706 cells suggest that γ-synuclein may play a role as a negative regulator in the development of human ESCC.

关 键 词：食道癌 磷状细胞癌 逆转录聚合酶链反应 Γ-SYNUCLEIN 

分 类 号：R735.1[医药卫生—肿瘤]