外周血甲胎蛋白mRNA和白蛋白mRNA在评估肝癌微小转移中的比较研究  

A comparative study on mRNA of AFP and albumin in peripheral blood as indicators in micro-metastasis of hepatocarcinoma cell

作　　者：智慧[1] 詹俊[2] 邓庆丽[3] 周雨迁[3] 邵静[3] 黄志明[3] 

机构地区：[1]中山大学孙逸仙纪念医院超声科,广州510120 [2]中山大学孙逸仙纪念医院消化内科,广州510120 [3]中山大学孙逸仙纪念医院医学研究中心,广州510120

出　　处：《临床检验杂志》2003年第5期259-261,共3页Chinese Journal of Clinical Laboratory Science

基　　金：97.3分课题资助项目 (G19990 5 410 5 )

摘　　要：目的　探讨肝病患者外周血甲胎蛋白mRNA(AFPmRNA)和白蛋白mRNA(ALBmRNA) ,作为肝癌微小转移标志物的特异性。方法　提取患者和健康志愿者的外周血单个核细胞总RNA ,进行RT PCR ,扩增产物再进行Southern杂交。结果　原发性肝癌 (HCC)组外周血AFPmRNA的检出率为 4 1.4 % ,对照组为 0 % ,差异显著 (P <0 .0 5 ) ;HCC组外周血ALBmRNA的检出率为4 4 .8% ,对照组为 2 9.2 % ,二者差异无统计学意义 (P >0 .0 5 )。结论　作为肝癌微小转移的标志物 。Objective To study the specificity of AFP mRNA and albumin mRNA (ALB mRNA)-positive cells in the peripheral blood as an indicator for circulating malignant hepatocyte.Method Using RT-PCR/Southern blot to detect the AFP mRNA and ALB mRNA in the peripheral blood from 29 patients with hepatocellular carcinoma (HCC) and 41 cases without HCC (8 of cirrhosis,4 of acute hepatitis,15 of chronic hepatitis,2 of hepatic angioma,2 of non-HCC hepatic tumor and 10 normal volunteer).Results In the patients with HCC,AFP mRNA was found in 12 of 29 cases (41.4%) and ALB mRNA was found in 13 of 29 cases (44.8%).In the control group,tAFPmRNA was not found,while ALB mRNA was found in 12 of 41 cases (29.2%).There was a significant difference for AFP mRNA positive rate between the two groups (P<0.05) and no significant difference for ALB mRNA positive rates between the two groups (P≥0.05).Conclusion As indicators of liver cancer cell in peripheral blood,AFP mRNA was more specific than ALB mRNA.

关 键 词：外周血 甲胎蛋白 mRNA 白蛋白 肝癌 微小转移 逆转录聚合酶链反应 Southem杂交 

分 类 号：R735.7[医药卫生—肿瘤]