SOD酶模型化合物对口腔颊黏膜细胞DNA的影响  

The Influence of MSOD on Buccal Cells'DNA

作　　者：于非非[1] 朱燕[1] 丁书茂[1] 鲁志松[1] 乔琰[1] 廖展如[1] 

机构地区：[1]华中师范大学,武汉430079

出　　处：《湖北预防医学杂志》2003年第4期4-6,共3页Hubei Journal of Preventive Medicine

基　　金：国家自然科学基金 ( 3 9470 63 2 )

摘　　要：目的　探讨超氧化物歧化酶模型化合物 (m odel of superoxide dism utase,MSOD) (Cu2 C2 1 N1 4H2 7Cl1 3)作用于正常口腔颊黏膜细胞的安全剂量范围。方法　用单细胞凝胶电泳法 ,通过观察 Tail Mom ent和Tail DNA %这两项指标的变化情况检测染毒后口腔颊黏膜细胞的 DNA损伤情况。结果　测试结果表明 2 5、5 0、1 0 0μg/ m l MSOD处理组相互之间、与阴性对照组之间均无显著性差异 ,但与阳性对照组之间有极显著性差异。结论　当 MSOD浓度 <1 0 0μg/ ml时 ,没有发现 MSOD对口腔颊黏膜细胞Objective To explore the safe range of the model of superoxide dismutase (MSOD)on normal buccal cells. Methods Using comets' tail moment and tail DNA% as parameter to detect the DNA migration of buccal cell by single cell gel electrophoresis(SCGE). Results The results show that there is no significant difference between MSOD group and control group, but the difference between MSOD and H 2O 2 is extremely significant. Conclusions MSOD doesnot cause DNA damage of human buccal cells if the concentration is below 100 μg/ml. [

关 键 词：超氧化物歧化酶模型化合物 口腔颊黏膜细胞 单细胞凝胶电泳 MSOD 

分 类 号：R781.5[医药卫生—口腔医学]