Primary nasopharyngeal non-Hodgkin lymphoma and its relationship with Epstein-Barr virus infection  

作　　者：张彬 宗永生 何洁华 钟碧玲 林素暇 

机构地区：[1]Department of Pathology,Sun Yat-sen Medical College,Sun Yat-sen University,Guangzhou 510089,China

出　　处：《Chinese Medical Journal》2003年第6期913-917,共5页中华医学杂志（英文版）

基　　金：This work was supported by the National Natural Science Foundationof China(No.39730200-Ⅱ).

摘　　要：Objectives To investigate the immunophenotypes of primary nasopharyngeal non-Hodgkin lymphoma (NPL) and their relationship to Epstein-Barr virus (EBV) infection.Methods The clinical data and biopsies of 73 patients with NPL were collected in Guangzhou. In situ hybridization was performed to detect the EBV-encoded small non-polyadenylated nuclear RNAs (EBERs) on biopsy slides. Immunohistochemistry was used to classify the immunophenotypes of NPL and detect EBV antigen expression.Results Forty-four (60.27%) of the 73 NPLs were of B cell lineage (CD79α+/CD3-/CD56-) while the 29 others (39. 73%) were of non-B cell lineage. Seventy-three NPLs could be classified into 3 major immunophenotypes: B cell (CD79α+/CD3-/CD56-, 44 cases), peripheral T cell (CD79α-/ CD3+/CD56-, 22) and NK/T cell (CD79α-/CD3+/CD56+, 7). The percentages of EBV infection differed among the 3 major immunophenotypes (B cell: 11. 36%, 5/44; peripheral T cell: 81. 82%, 18/22; NK/T cell: 100% ,7/7). Both CD56-ositive and CD56-negative immunophenotypes could further be divided into 4 subtypes; CD8-/CD4-, CD8+/CD4-, CD8-/CD4+ and CD8+/CD4 + . All the CD8-/CD4- NPLs with CD56-positivity (7) or CD56-negativity (2) were infected with EBV. The neoplastic cells of a nasopharyngeal Burkitt' s lymphoma expressed EBV nuclear antigen 1 (EBNA1) and EBV RNA (EBERs) only. In the other 29 EBV-infected NPLs, most of the lymphoma cells harboring EBV also expressed EBNA1 and EBERs; 21 of the 29 NPLs had a considerable number of neoplastic cells expressing latent membrane protein 1 (LMP1) (21/29, 72. 41 %) and 23 of 29 NPLs expressed latent membrane protein 2A (LMP2A) (23/29, 79. 31%). A few lymphoma cells in 17 (17/29, 58. 62%), 23 (23/29, 79. 31 %) and 22 NPLs (22/29, 75. 86%) expressed Zta (Bam HI Z transactivator), viral capsid antigen (VGA) and membrane antigen (MA), respectively.Conclusions The prevalence ratio of the 3 immunophenotypes, namely, B cell, peripheral T cell and NK/T cell lymphoma, is about 6:3:1. However, the EBV infection ratio is reversed, 1:8:1Objectives To investigate the immunophenotypes of primary nasopharyngeal non-Hodgkin lymphoma (NPL) and their relationship to Epstein-Barr virus (EBV) infection.Methods The clinical data and biopsies of 73 patients with NPL were collected in Guangzhou. In situ hybridization was performed to detect the EBV-encoded small non-polyadenylated nuclear RNAs (EBERs) on biopsy slides. Immunohistochemistry was used to classify the immunophenotypes of NPL and detect EBV antigen expression.Results Forty-four (60.27%) of the 73 NPLs were of B cell lineage (CD79α+/CD3-/CD56-) while the 29 others (39. 73%) were of non-B cell lineage. Seventy-three NPLs could be classified into 3 major immunophenotypes: B cell (CD79α+/CD3-/CD56-, 44 cases), peripheral T cell (CD79α-/ CD3+/CD56-, 22) and NK/T cell (CD79α-/CD3+/CD56+, 7). The percentages of EBV infection differed among the 3 major immunophenotypes (B cell: 11. 36%, 5/44; peripheral T cell: 81. 82%, 18/22; NK/T cell: 100% ,7/7). Both CD56-ositive and CD56-negative immunophenotypes could further be divided into 4 subtypes; CD8-/CD4-, CD8+/CD4-, CD8-/CD4+ and CD8+/CD4 + . All the CD8-/CD4- NPLs with CD56-positivity (7) or CD56-negativity (2) were infected with EBV. The neoplastic cells of a nasopharyngeal Burkitt' s lymphoma expressed EBV nuclear antigen 1 (EBNA1) and EBV RNA (EBERs) only. In the other 29 EBV-infected NPLs, most of the lymphoma cells harboring EBV also expressed EBNA1 and EBERs; 21 of the 29 NPLs had a considerable number of neoplastic cells expressing latent membrane protein 1 (LMP1) (21/29, 72. 41 %) and 23 of 29 NPLs expressed latent membrane protein 2A (LMP2A) (23/29, 79. 31%). A few lymphoma cells in 17 (17/29, 58. 62%), 23 (23/29, 79. 31 %) and 22 NPLs (22/29, 75. 86%) expressed Zta (Bam HI Z transactivator), viral capsid antigen (VGA) and membrane antigen (MA), respectively.Conclusions The prevalence ratio of the 3 immunophenotypes, namely, B cell, peripheral T cell and NK/T cell lymphoma, is about 6:3:1. However, the EBV infection ratio is reversed, 1:8:1

关 键 词：nasopharynx·lymphoma non-Hodgkin·immunophenotype ·Epstein-Barr virus 

分 类 号：R739.63[医药卫生—肿瘤] R373[医药卫生—临床医学]