儿童非霍奇金淋巴瘤EB病毒LMP-1和P^(53)、bcl-2表达的关系  

Clinicophatological features of non-hodgkin's lymphoma in children and the relationship of LMP-1 and P^(53)、bcl-2 protein expression

作　　者：黄波[1] 郭瑞珍[2] 明晓务 王俊[1] 李青[1] 唐文台[2] 肖庆帮[2] 

机构地区：[1]遵义市妇女儿童医院,563000 [2]遵义医学院病理教研室,563003

出　　处：《贵州医药》2003年第10期867-871,共5页Guizhou Medical Journal

摘　　要：目的 探讨儿童NHL EB病毒LMP-1和P^(53)、bcl-2蛋白的表达及关系。方法 采用免疫组化Envision法检测64例儿童NHL中LMP-1和P^(53)、bcl-2蛋白。结果(1)P^(53)蛋白阳性表达39例(60.9％),表达强度与淋巴瘤恶性程度呈正相关;阳性表达率在低恶组与中、高恶性组间有显著性意义,P<0.01。bcl-2蛋白阳性表达37例(53.8％),bcl高于TCL,低恶性高于高恶性。(2)LMP-1蛋白阳性表达45例(70.3％),阳性表达率与肿瘤恶性程度和年龄有统计学意义,P<0.01;而与淋巴瘤免疫表型、性别和发病部位无关。LMP-1表达与P^(53)及bcl-2的表达呈正相关。结论 EBV感染是儿童NHL发生发展不可忽视的病毒致病因素,其致病作用可能是通过上调P^(53)、bcl-2蛋白实现的。Objective To study the clinicophatological features of Non-Hodgkin's lymphoma in children and the relationship of LMP-1 and p53、 bcl-2 protein expression. Methods The expression of P53、bcl-2、LMP-1 protein in children's NHL were detected with immunohistochemical method. Results The expression of P53 were detected in 39(60. 9%) of 64 cases of NHL in children, which positively correlated with the aggressiveness of lymphomas. And there was a great significance between the expression of P53 in lowly aggressive lymphoma and in intermediately aggressive lymphoma and highly aggressive ones. The expression of bcl-2 were detected in 37 (53. 8%) of 64 cases, the expression of bcl-2 was higher in BCL than TCL, in low-grade than high-grade lymphoma. (4) The protein of LMP-1 was positive in 45/64 cases of NHL, and the expression closely correlated with the aggressiveness and age of cases, but has no pertinence the immune phenotype,gender and lesion site of NHL. However, the expression of LMP-1 were positively relevant to the expression of P53 and bcl-2 in NHL. Conclusion The infection of EBV might still play an important role in the pathogensis and development of NHL in children, mainly through inducery the overexpression of P53 and bcl-2.

关 键 词：儿童 非霍奇金淋巴瘤 EB病毒 LMP-1 P^53 bcl-2 表达 

分 类 号：R733.1[医药卫生—肿瘤]