毒物代谢酶基因多态性与结肠腺癌易感性关联  

Relation between genetic polymorphism in xenobiotica metabolizing enzymes and risk of sporadic colorectal adenocarcinoma

作　　者：邓长生[1] 张友才[1] 林军[1] 孙洁[1] 

机构地区：[1]武汉大学中南医院消化内科,430071

出　　处：《中华消化杂志》2003年第9期540-543,共4页Chinese Journal of Digestion

摘　　要：目的　探讨谷胱甘肽S 转移酶 (GST)M 1、T1和N 乙酰化转移酶 1(NAT1)基因多态与散发性结肠腺癌 (SCRAC)遗传易感性的关联。方法　应用PCR 限制性片段长度多态性 (RFLP)及多重PCR技术检测GSTM 1、GSTT1和NAT1基因多态性。结果　GSTM1、GSTT1空白基因型在对照组与病例组之间的频率比较 ,差异均无显著性 ,而NAT1 10的频率差异有显著性 (2 7.8%∶4 2 .3% ,P <0 .0 5 )。不同临床特征、老年或非老年SCRAC与对照组GSTM 1空白基因型的频率比较 ,差异均无显著性 ;GSTT1空白基因型在SCRAC远端 (6 6 .2 % ,P <0 .0 5 )、DukesC期 (80 .8% ,P <0 .0 1)及低分化(77.1% ,P <0 .0 1)中的频率均显著高于对照组 (4 7.5 % )。NAT1 10在不同部位SCRAC与对照组之间的频率差异无显著性 ,SCRAC在老年 (5 2 .7% ,P <0 .0 1)、DukesC期 (5 3.8% ,P <0 .0 5 )及中分化(5 4 .2 % ,P <0 .0 1)中的频率均显著高于对照组。结论　GSTT1空白基因型与远端SCRAC易感性有关 ,癌肿多处于DukesC期 ,多呈低分化腺癌 ;NAT1 10与SCRAC的遗传易感性有关 ,癌肿多见于老年患者 ,多处于DukesC期 ,多呈中度分化腺癌。Objective To analyze the association of genetic polymorphism of Glutathione S transferase (GST) M1, T1 and N acetyltransferase 1(NAT1) with genetic susceptibility to sporadic colorectal adenocarcinoma (SCRAC). Methods All subjects were Han people in Hubei province of China. Using multiple PCR and PCR RFLP, we studied the genetic polymorphism of the GSTM1, GSTT1 and NAT1 genes. Results There were no significant differences of the frequency of GSTM1 and GSTT1 null genotype between controls and SCRAC except for the differences of frequency of NAT1 *10 (27.8%∶42.3%, P <0.05), and there were no significant differences of the frequency of GSTM1 null genotype between controls and proximal or distal SCRAC, different Dukes stages, different differentiated SCRAC and the elder or younger SCRAC respectively. The frequency of GSTT1 null genotype was more common in distal SCRAC (66.2%, P <0.05), among SCRAC in Dukes C stage (80.8%, P < 0.01 ) and in poorly differentiated SCRAC (77.1%, P < 0.01 ) when compared with the controls (47.5%). There were no significant differences of the frequency of NAT1 *10 between proximal or distal SCRAC and controls, but the frequency of NAT1 *10 null genotype was more common in the elder SCRAC(52.7%, P <0.01), among SCRAC in Dukes C stage (53.8%, P < 0.05 ) and in moderately differentiated SCRAC(54.2%, P <0.01) when compared with the controls. Conclusions The GSTT1 null genotype may influence the distal SCRAC, and most tumors are in Dukes C stage and poorly differentiated SCRAC. NAT1 *10 may also increase the risk of SCRAC, especially in the elder, and most tumors are in Dukes C stage and moderately differentiated SCRAC.

关 键 词：毒物代谢酶 基因多态性 结肠腺癌 易感性 

分 类 号：R735.35[医药卫生—肿瘤]