复方中药99-克星超声介入治疗肝癌裸鼠移植瘤凋亡与增生  

Apoptosis and proliferation of hepatocellular carcinoma in nude mice with percutaneous intratumor injection of Chinese herbal medicine "Star-99"

作　　者：林晓东 林礼务 何以敉 高上达 杨发端[2] 薛恩生 

机构地区：[1]福建省超声医学研究所 [2]福建医科大学附属协和医院病理科,福建省福州市350001

出　　处：《世界华人消化杂志》2003年第9期1353-1356,共4页World Chinese Journal of Digestology

基　　金：福建省科委基金;No.2000Z138

摘　　要：目的:探讨超声引导瘤内注射复方中药99-克星治疗肝癌SMMC-7721裸鼠移植瘤的抗癌作用.方法:取人肝癌SMMC-7721裸鼠皮下移植模型28只,随机分为99-克星中药治疗组14只,无水乙醇与生理盐水治疗组各 7只.全部裸鼠在接种 10 d后每隔 5 d向肿瘤内注入治疗药物共 4次,治疗 20 d后处死.应用免疫组织化学方法检测移植瘤细胞Ki-67抗原的表达并行流式细胞仪(FCM)DNA分析以及采用TUNEL方法对移植瘤细胞凋亡现象进行观察.结果:中药99克星组与乙醇组的Ki-67指数分别为17±9％、21±11％,均显著低于盐水组的32 ±13％(P<0.05);克星组凋亡指数高达49 ±5％,显著高于乙醇组的12±2％和盐水组的10±4％(均P<0.01);FCM DNA分析显示盐水组的异倍体峰出现率高达57％,明显高于克星组的7％与乙醇组的0％(P均<0.05),而克星组的凋亡峰出现率高达93％,明显高于乙醇组的 14％与盐水组的0％(P均<0.01).结论:复方中药99克星能显著抑制裸鼠移植瘤细胞的增生,并诱发其凋亡.研究结果表明复方中药99克星具有强大的破坏与抑制肿瘤细胞生长的作用,其疗效不亚于无水乙醇,其主要机制可能有:(1)抑制癌细胞的增生,(2)诱导癌细胞凋亡.复方中药99-克星抗肿瘤的作用机制不同于无水乙醇,尤其以抑制癌细胞增生和诱导癌细胞凋亡的作用机制在抗肿瘤中具有重要的意义.本研究为传统中药治疗肝癌提供了一个新的给药途径与理论依据,其在肿瘤治疗研究中具有重要的意义和良好的发展前景。AIM: To probe the anti-cancer effect of ultrasound-guided local injection with Chinese traditional medicine 'Star-99' in the treatment of hepatocellular carcinoma (HCC). METHODS: Twenty-eight human hepatocellular carcinoma SMMC-7721 transplantation nude mice were randomly divided into Star-99 group containing (14 patterns), ethanol group (7 patients) and saline group (7 patterns ). Ten days after transplantation, the medicine was injected into the tumors of all the nude mice every 5 days. On day 20 after the first injection, the nude mice were killed. Immunohistochemistry assay was adopted to detect the expression of Ki-67 antigen in HCC, The tumor tissues were sent for flow cytometry (FCM) and DNA analysis. Apoptotic cells were visualized by TUNEL assay. All the experiments were carried out under double blind. RESULTS: The Ki-67 Labeling index in Star-99 group (17±9 %) and ethanol group (21±11 %) was significantly lower than that in saline group (32±13 %) (P<0.05). The mean apoptotic index (AI: percentage of TUNEL signal positive cells) in Star-99 group (49±5 %) was significantly higher an that in ethanol group (12±2 %) and the saline group (10±4%) (P<0.01). FCM and DNA analysis showed that the appearance rate of the apoptosis peak in Srar-99 group was 93 %, markedly higher than that in ethanol group (14 %) and saline group (0 %) (P<0.01). CONCLUSION: Star-99 can inhibit cancer cells proliferation remarkably and induce the cancer cell apoptosis. It has strong effects of inhibiting and destructing cancer cells. Its curative effect is as good as the ethanol. Its major mechanisms can be as follows: (1) It can inhibit cancer cell proliferation. (2) It can induce cancer cell apoptosis. The mechanism of Star-99 is different from that of ethanol. The mechanisms of inhibiting cancer cell proliferation and inducing cell apoptosis is of great significance. This study will provide a new pathway for drug administration and an experimental basis for the treatment of HCC with tradi- tional Chinese herbal. The study of S

关 键 词：复方中药 99-克星 超声介入 中医药疗法 肝癌 裸鼠移植瘤 细胞凋亡 细胞增生 肿瘤内注射 

分 类 号：R735.7[医药卫生—肿瘤]