TRAIL诱导肝癌细胞系SMMC-7721的凋亡作用  

Role of TRAIL in inducing apoptosis of SMMC-7721 cells

作　　者：李小安[1] 房殿春[2] 司佩任[2] 张汝刚[2] 杨柳芹[2] 秦建平[1] 

机构地区：[1]中国人民解放军成都军区总医院消化内科,四川省成都市610083 [2]中国人民解放军第三军医大学西南医院全军消化专科中心,重庆市400038

出　　处：《世界华人消化杂志》2003年第9期1369-1371,共3页World Chinese Journal of Digestology

基　　金：全军"十五"科研基金;No.01MA172

摘　　要：目的:观察TRAIL诱导肝癌SMMC—7721细胞凋亡的作用.方法:采用MTT法检测细胞存活分数;TUNEL法检测细胞凋亡率;流式细胞仪检测细胞凋亡和细胞周期;电镜观察凋亡细胞超微结构.结果:TRAIL对SMMC-7721细胞的存活分数和凋亡率的影响呈典型的量效关系,经TRAIL作用后的细胞,流式细胞仪检测呈标准的亚二倍峰,电镜观察发现经TRAIL作用的部分细胞具有凋亡细胞的典型形态特征.结论:TRAIL可诱导SMMC—7721细胞凋亡.AIM: To observe the role of TRAIL in inducing apoptosis of SMMC-7721 cells. METHODS: The survival fraction of SMMC-7721 cells was measured by MTT assay; Apoptosis rate was determined by TUNEL method and the ultramicrostructure of apoptosic cells induced by TRAIL was observed by electron-microscopy. RESULTS: The role of TRAIL in survival fraction and apoptosis rate demonstrated a good relationship and the typic structure of apoptosic cells was foud in some cells treated by TRAIL. CONCLUSION: TRAIL can induce apoptosis in SMMC-7721 cells.

关 键 词：TUNEL法 细胞凋亡 肝癌 SMMC—7721细胞系 细胞周期 流式细胞仪 电镜 TRAIL基因 

分 类 号：R735.7[医药卫生—肿瘤]