急慢性淋巴细胞白血病免疫表型分析  

Analysis of the immunophenotype of lymphocytic leukemia

作　　者：高原[1] 刘家应[1] 倪麟[2] 陈松立[2] 

机构地区：[1]上海市儿童医院检验科,上海200040 [2]上海市第二医科大学附属瑞金医院检验系

出　　处：《中国实验诊断学》2003年第5期386-388,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　探讨急性淋巴细胞白血病 (ALL)和慢性淋巴细胞白血病 (CLL)细胞免疫表型差异。方法　应用ABC AP免疫组织化学方法 ,选用一系列的淋巴细胞相关抗原的单抗对 16 9例初发ALL与 38例CLL患者进行淋巴细胞表面抗原检测。结果　B ALL患者白血病细胞的CD19、HLA DR阳性率较高 ,T ALL患者的白血病细胞以CD7、CD5与CD3 阳性率较高 ,CLL患者的白血病细胞除了以CD19、CD2 0 、CD2 2 阳性率较高外 ,CD5也有明显表达。结论　对淋巴细胞白血病患者进行免疫表型分析 ,对于区别细胞类型 ,鉴别诊断有重要意义 ,为临床治疗提供依据。Objective To study the difference in expressing the immunophenotype of acute lymphocytic leukemia(ALL) and chronic lymphocytic leukemia(CLL).Methods The immunophenotypes of 169 ALL cases and 38 CLL cases were determined by a panel of monoclonal antibodies with avidin-biotin-immunoalkaline-phosphatase(ABC-AP) method. Results CD 19 , HLA-DR were highly expressed in B-ALL while CD 7,CD 5 and CD 3 were highly expressed in T-ALL.In CLL,the expression of CD 5 was higher than that of CD 19 ,CD 20 and CD 22 . Conclusion The immunotyping technique is very effective in diagnosing lymphocytic leukemia and may be help for therapy.

关 键 词：急性淋巴细胞白血病 慢性淋巴细胞白血病 免疫表型 抗原 免疫组织化学 

分 类 号：R733.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]