机械压力对青春期大鼠咀嚼肌细胞增殖活性影响的体外研究  

Study on Mechanical Compression Regulating the Proliferation of Young Growing Rat Masticatory Myocyte in vitro

作　　者：陈开云[1] 罗颂椒[2] 

机构地区：[1]广东省口腔医院正畸科,510280 [2]四川大学华西口腔医院正畸科

出　　处：《华西口腔医学杂志》2003年第5期403-405,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (编号 30 1 71 0 2 4 )

摘　　要：目的　建立咀嚼肌细胞的力学刺激—细胞培养模型 ,在细胞水平探讨机械力作用下青春期大鼠咀嚼肌细胞功能代谢的变化。方法　采用流式细胞术检测不同时段、不同力值的机械压力对体外培养的青春期大鼠咀嚼肌细胞增殖活性的影响。结果　各实验组肌细胞的DNA含量 (S期百分数 )均大于对照组。细胞持续受力 2h后 ,实验组细胞的增殖指数 (PI)较对照组高 ;细胞持续受力 4h后 ,在 2 0 0 0 μstrain组的细胞增殖指数达到最高(4 8 9% ) ,而 4 0 0 0 μstrain组的PI在所有实验组中最低 (39 0 % )。 结论　适当的压力对咀嚼肌细胞起着促进增殖的作用 ;而较大的压力作用较长时间时 。Objective\ To establish an experimental model which was the masticatory myocyte culture and force stimulation in vitro and study the proliferation changes of cultured masticatory myocytes caused by mechanical compression in young rat. Methods\ Flow cytometry (FCM) was used to examine the changes of cellular DNA content and cell cycles of cultured masticatory myocytes. Results\ The DNA content in experimental groups was higher than that in control. After the compressive force was applied for 2 hours, the proliferation index (PI) in experimental group became higher than that in control. Under a continuous pressure for 4 hours, the PI in 2 000 μstrain group reached the maximum (48 9%) but the PI in 4 000 μstrain group reached the minimum (39 0%).Conclusion\ The proliferation of masticatory myocytes from young rat increased under certain force and certain period of time, but decreased if the force applied was overloaded.

关 键 词：机械压力 青春期 大鼠 咀嚼肌细胞 细胞增殖活性 牙颌面畸形 

分 类 号：R783.5[医药卫生—口腔医学]