超氧阴离子自由基诱导皮层神经细胞Cu/ZnSOD基因表达的研究  

Superoxide Anion-induced SOD Gene Expression on the Cultured Cerebral Cortex Neuron of Neonate Rat

作　　者：姜招峰[1] 何湘平[2] 刘传缋[2] 杨翰仪[3] 

机构地区：[1]北京联合大学应用文理学院,北京100083 [2]军事医学科学院毒物药物研究所,北京100850 [3]吉林大学医学部生物化学教研室,长春130021

出　　处：《北京联合大学学报》2003年第3期9-11,共3页Journal of Beijing Union University

摘　　要：　目的:探讨超氧阴离子自由基对神经细胞Cu ZnSOD基因表达的影响。方法:以产超氧阴离子自由基系统(XO X)作用于原代培养的新生大鼠大脑皮层神经元,分析SOD含量、SOD活性和SODmRNA丰度。结果:SOD含量随超氧阴离子自由基作用时间的延长显著增加,第5天后增加的变化趋于稳定,第7天达每毫克蛋白质(1 24±0 23)μg;SOD活性在开始的1～5d呈上升状态,第5天为每毫克蛋白质(5 30±0 44)U,第6天开始显著下降为每毫克蛋白质(2 15±0 32)U,且稳定至第7天的每毫克蛋白质(2 08±0 73)U;SODmRNA的相对丰度显著高于对照组。结论:超氧阴离子自由基可诱导培养的神经细胞Cu ZnSOD基因表达;SOD活性变化和SOD含量变化并不平行,可能是在超氧阴离子自由基作用一定时间后,细胞内出现了无活性或 和低活性的SOD。Aim: To elucidate the effects of superoxide anion on superoxide dismutase (SOD) in the cultured cerebal cortex neuron of neonate rat Methods: With Xanthine Oxidase/Xanthine (XO/X) was used as the source of the superoxide anion, to study on SOD activity and SOD content and SODmRNA content Results: In the XO/Xtreated groups, SOD contents increased to 124±023?μg/mg/protein after 6th day; from first day to fifth day, SOD activities increased, and to 530±044 U/mg/protein after 5th day; from 6th day SOD activities decreased, and to 208±073U/mg/protein after 6th day; SODmRNA contents increased at 7th day Conclusion: Superoxide anion can induce SOD gene expression in the neural cells; SOD activity and SOD content do not parallel from after 5th day with XO/X treated, maybe it is occurred that SOD changes into the less activity form or/and the no activity form

关 键 词：超氧阴离子自由基 诱导 皮层神经细胞 Cu/ZnSOD 基因表达 活性 老年性痴呆 氧化修饰 

分 类 号：R749.16[医药卫生—神经病学与精神病学]