pH值对端粒酶基因反义核苷酸促进ADM、DDP抑制人肝癌细胞BEL-7404增殖的影响  

The effect of pH on the human telomerase reverse transcriptase gene antisense oligodeoxynucleotide enhancing the killing role of ADM and DDP to hepatic cancer cells

作　　者：吴成荣 范平生[2] 

机构地区：[1]马钢医院干部病房,安徽马鞍山243000 [2]安徽省立医院分院肿瘤科,合肥230031

出　　处：《安徽医科大学学报》2003年第5期342-344,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省重点科研项目资助课题 (编号 :0 10 2 3 0 2 7)

摘　　要：目的　观察不同 pH值对人类端粒酶逆转录酶(hTERT)基因反义寡核苷酸 (ASODN )促进盐酸阿霉素(ADM)、顺铂 (DDP)抑制人肝癌细胞增殖作用的影响。方法　 96孔培养板上进行增殖抑制实验 ,用不同pH值的RP MI 16 4 0培养液的BEL 74 0 4细胞悬液接种于 96孔细胞培养板 ,第 1天加端粒酶基因的反义寡核酸 5 μmol/L ,2 4、4 8h后再分别加入 2 5 μmol/L ,2 4h分别加入DDP、ADM5 μmol/L。各 pH值下分设hTERT基因的ASODN对照组、DDP组、hTERT基因的ASODN +DDP组、ADM组、hTERT基因的ASODN +ADM组、BEL 74 0 4细胞对照组 ,每组各设 8个复孔 ,置 37℃恒温箱培养 4dMTT法测定结果。结果　在培养液pH 6 8时 ,hTERT基因的ASODN能提高DDP和ADM对肿瘤细胞增殖抑制作用 ,在 pH 7 3培养液时 ,hTERT基因的ASODN更能提高DDP和ADM对肿瘤细胞增殖抑制作用更强。结论　在体外 ,pH值对hTERT基因的ASODN促进ADM、DDP抑制BEL 74 0 4细胞增殖有一定的影响 ,hTERT基因的ASODN促进ADM、DDP抑制BEL 74 0 4细胞的作用需有培养液的最适Objective To study the effect of pH on the hu ma n telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide ( ASODN) in enhancing the killing role of ADM and DDP to hepatic cancer cells. Methods RPMI-1640 culture fluids with variable pH were used to modify the BEL-7404 cel ls to the concentration of 4×10 6/L in inocunated 96 hole of a culture board. The hTERT gene ASODN of 5 μmol/L was added on the first day and at 24 h and 48 h, the same agent of 2 5 μmol/L was added again respectively .At 24h ADM and DDP were added .Under variable pH the hTERT gene ASODN group, DDP group, hTERT gene ASODN + DDP group, ADM group, hTERT gene ASODN +ADM group and BEL-7404 ce lls group were set up using eight holes for each group. The board was put in a 3 7℃ constant temperature chest for four days and the results were tested with MT T. Results The hTERT gene ASODN, enhanced apparently the effect o f ADM and DDP in killing BEL-7404 cells at pH 7 3,and at pH 6 8 the hTERT ge ne ASODN increased the killing rate of both ADM and DDP. Conclusion In vitro, different pH has different effect on the hTERT gene ASODN in enhancing the killing role of ADM and DDP to BEL-7404 cells.

关 键 词：端粒酶 PH值 反义核苷酸 ADM DDP 肝癌 细胞增殖 阿霉素 顺铂 

分 类 号：R735.7[医药卫生—肿瘤] R979.14[医药卫生—临床医学]