傅里叶变换红外光谱用于食管癌及癌旁正常组织结构的相关研究  

FTIR Spectroscopic Study on Normal and Cancerous Tissues of Esophagus

作　　者：王健生[1] 徐怡庄[1] 石景森[2] 张莉[1] 段小艺[2] 杨丽敏[3] 苏允兰[3] 翁诗甫[3] 徐端夫[4] 吴瑾光[1] 

机构地区：[1]北京大学化学与分子工程学院化学生物学系,北京100871 [2]西安交通大学第一医院,陕西西安710061 [3]北京大学化学与分子工程学院,化学生物学系北京100871 [4]中国科学院化学所,北京100080

出　　处：《光谱学与光谱分析》2003年第5期863-865,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(3973016);国家重点基础研究发展规划项目(G1998061307);科技部前期研究专项2002 CCA01900资助

摘　　要：应用傅里叶变换红外光谱法(FTIR)对43例食管癌及癌旁正常组织进行了分析,结果表明,食管癌组织和癌旁正常组织的红外光谱具有较大的差异:正常组织的红外光谱在1550 cm-1附近的酰胺Ⅱ带吸收峰相对较强,峰型高尖,与酰胺Ⅰ带的相对峰高比(I1647/I1550)在癌组织中比较高;正常组织中酰胺Ⅰ带的吸收峰峰位比癌组织的高1-6 cm-1;正常组织中1453 cm-1处的峰强大于1402 cm-1处,而在癌组织中则相反,1402 cm-1附近的吸收峰峰高大于1453cm-1处;癌组织中1080 cm-1附近的峰位和正常组织相比显得高而强,癌组织的相对峰高比(I1080/I1550)高于正常组织。说明傅里叶变换红外光谱法可以用于对良、恶性组织进行鉴别诊断,有希望发展成为肿瘤快速诊断的一种新方法。Fourier transform infrared spectroscopy (FTIR) was applied to the study of cancerous tissues and normal tissues near the cancers of 43 esophagus cancer patients. The results showed that significant differences were seen in the FTIR spectra between the normal and malignant esophageal tissues. The amide band II at about 1550 cm(-1) was weak and broad in the malignant tissues but it was strong and sharp in the normal tissues; the relative intensity of I-1647/II1550 of the malignant tissues was higher than that of the normal tissues; the peak wavenumber of the normal tissues was about 1-6 cm(-1) higher than that of the malignant tissues; the intensity of the band at 1453 cm(-1) in the normal tissues was higher than that in the malignant tissues; the band at 1080 cm(-1) was higher and stronger in the malignant tissues than that in the normal tissues. The ratio of I-1080/I-1550 was higher in the malignant tissues. This might indicate that the FTIR could be used in the clinical diagnoses.

关 键 词：傅里叶变换红外光谱 食管癌 癌旁正常组织 结构 恶性组织 鉴别诊断 

分 类 号：R735.1[医药卫生—肿瘤] O657.33[医药卫生—临床医学]