绿色荧光蛋白基因在根际优势菌株中的高效表达  

High Expression of EGFP Gene in Activity Bacteria of Jute Rhizosphere

作　　者：陈菲莉[1] 张甲耀[1] 方呈祥[1] 季淼[1] 陈玲[1] 邓欢欢[1] 陈效[1] 

机构地区：[1]武汉大学资源与环境科学学院,武汉430072

出　　处：《环境科学与技术》2003年第6期1-2,5,共3页Environmental Science & Technology

基　　金：国家自然科学基金项目 (2 0 0 770 2 1 )

摘　　要：从人工模拟污染土壤中的黄麻根际分离得到两株优势菌株。通过PCR技术对增强绿色荧光蛋白基因与质粒载体pTnMod Ocm进行体外重组构建新的质粒 ,并转化分离得到的优势菌株。Two activity strains were screened from the roots of jute which grew in polluted soil. Enhanced green fluorescent protein (EGFP) gene obtained by means of PCR was combined in vitro with the plasmid pTnMod-Ocm which contains mini-Tn5. Then the recombined plasmids pTnMod-Ocm-EGFP was transformed into Tu-1 successfully. It has been showed that EGFP gene was highly expressed in Tu-1/ pTnMod-Ocm-EGFP based on the fluorescent character.

关 键 词：增强绿色荧光蛋白 基因克隆 高效表达 

分 类 号：X172[环境科学与工程—环境科学]