多发性骨髓瘤患者EB病毒及人类疱疹病毒6感染的研究  

Detection of virus DNA in multiple myeloma patients

作　　者：王京华[1] 刘娜[1] 刘芳[1] 郭博[1] 

机构地区：[1]哈尔滨医科大学附属第二医院血液病研究所,哈尔滨150086

出　　处：《临床血液学杂志》2003年第6期252-254,共3页Journal of Clinical Hematology

基　　金：黑龙江省自然科学基金资助项目 (D 0 0 4 5 )

摘　　要：目的 :研究黑龙江地区多发性骨髓瘤 (MM )患者EB病毒 (EBV)、人类疱疹病毒 6 (HHV 6 )的感染情况 ,以探究MM的病因及发病机制。方法 :用多聚酶链反应 (PCR)技术检测MM患者EBV、HHV 6感染情况 ,每批试验均设阳性质粒对照及无模板阴性对照 ,同时检测了 2 6例正常人。结果 :MM患者总的病毒感染率为83.3% ,EBV DNA阳性率为 5 0 .0 % ,正常对照组EBV DNA阳性率为 3.8% ;MM患者HHV 6DNA阳性率为33.3% ,正常对照组HHV 6DNA为 0。MM患者EBV、HHV 6感染与正常对照组差异有极显著性意义 (P <0 .0 1)。结论 :黑龙江地区一些MM患者存在EBV、HHV 6感染 ,但两种病毒致病的确切机制尚不清楚。Objective:To study the infection stations of EBV and HHV-6 of Multiple myeloma patients in Hei Long Jiang, and to explore the etiology and mechanism.Method:The infection of EBV and HHV-6 of MM was detected by PCR. Every experiment had postive plasmid control and no template negative control and also were detected twenty-six normal persons.Result:The total infective rate of the virus in MM patients was 83.3%, the postive rate of the EBV was 50.0%, and the postive rate of EBV in normal control group was 3.8%. The postive rate of HHV-6 DNA in MM patients was 33.3%, and the postive rate of HHV-6 DNA of the normal control groop was zero. The comparision between MM patients and normal control group infected by EBV and HHV-6 has significant difference(P< 0.01).Conclusion:The infection of EBV and HHV-6 in MM patients in Hei Long Jiang is postive in certain degree,but the incisive mechanism was not clear.

关 键 词：骨髓瘤 多发性 多聚酶链反应 EB病毒 人类疱疹病毒6 

分 类 号：R733.3[医药卫生—肿瘤]