IFN-γ转基因治疗与肿瘤内注射重组IFN-γ对荷瘤小鼠疗效的比较  

A Comparison Study of Effect on Transgenic Therapy of IFN-y Gene and Therapy of Intratumoral Injection of Recombinant IFN-y in Tumor-bearing Mice

作　　者：严鹏霄[1] 丁强[1] 沈历宗[1] 范萍[1] 吴文溪[1] 

机构地区：[1]南京医科大学第一附属医院胃肠外科

出　　处：《南京医科大学学报（自然科学版）》2003年第6期550-553,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学创新基金(CS9905)

摘　　要：目的;评价IFN-γ转基因方法与肿瘤内注射重组IFN-γ对荷瘤小鼠的治疗作用。方法:用小鼠结肠癌细胞CT26接种于Balb/c小鼠皮下,建立小鼠皮下种植模型;小鼠成瘤后分成5组(每组9只),分别进行不同的干预。①组1 以阳离子脂质体为载体将真核表达质粒pcDNA3-IFN-γ导入结肠癌肿块细胞;②组2 肿瘤内注射重组IFN-γ,每日1次,连续4周;③组3 肿瘤内注射重组IFN-γ,每周3次,连续4周;④组4 为生理盐水对照组;⑤组5 为空载质粒对照组。结果:与生理盐水对照组比较,转基因治疗组和外源IEN-γ给药组抑瘤效应明显;组1、2、3荷瘤小鼠平均生存期分别为(67.3±4.7)天、(65.8±4.3)天及(57.5±4.4)天,明显长于组4(42.3±2.4)天与组5(41.0±2.1)天。结论:IFN-γ转基因治疗对荷瘤小鼠的肿瘤生长有抑制效应,转基因治疗组与重组IFN-γ每日给药组抑瘤效果相当,而优于重组IFN-γ间断给药组。Objective: To evaluate the effects on transgenic therapy of IFN-7 gene and therapy of intratumoral injection of recombinant IFN--y in tumor-bearing mice. Methods: Balb/c mice were used as animal model with subcutaneous inoculation of colon cancer cell of CT26. The tumor-bearing mice were divided into several groups (nine mice in each group), receiving intervention with different methods. Group 1 animals were treated with liposome-mediated eukaryotic expression plasmid of pcDNA3-IFN-'y; Group 2 with intratumoral injection of recombinant IFN-'y daily for 4 weeks; Group 3 with injection of IFN--y 3 times per week for 4 weeks; Group 4 with daily saline injections and Group 5 with empty plasmid as control. Results: Tumor regression was significant in group 1 and 2. The mean survival time of tumor-bearing mice in group 1, 2, 3 were(67. 3 +4. 7) days, (65. 8 +4. 3) days and (57. 5 +4. 4) days respectively , which were significantly longer than that in group 4(42. 3 + 2. 4) days. Conclusion: There is inhibition of tumor growth effect in tumor-bearing mice treated with transgenic therapy of IFN-'y gene. The efficacy of transgenic therapy of IFN-'/ gene is the same as the group of the group of daily intratumoral injection of rIFN-7, but is better than that of the group of intermittent r!FN--y injection.

关 键 词：结肠肿瘤 Γ-干扰素 基因治疗 小鼠 

分 类 号：R735.35[医药卫生—肿瘤] Q785[医药卫生—临床医学]