腺病毒介导CD/5-FC系统治疗口腔鳞癌机制的研究  

The therapeutic mechanism of adenovirus mediated CD/5-FC system in the treatment of oral squamous carcinoma cells

作　　者：王安训[1] 黄洪章[2] 

机构地区：[1]中山大学附属第一医院口腔科,510080 [2]中山大学附属第二医院口腔颌面外科

出　　处：《实用口腔医学杂志》2003年第5期435-438,共4页Journal of Practical Stomatology

基　　金：中山医科大学"2 1 1工程"基金 (990 74);广州市科技计划项目(2 0 0 1J0 2 1 0 1 )资助

摘　　要：目的 :研究腺病毒介导的大肠杆菌胞嘧啶脱氨酶基因 (AdCMVCD) /5 氟胞嘧啶 (5 FC)自杀基因治疗口腔鳞癌的作用机制。方法 :AdCMVCD/5 FC系统治疗口腔鳞癌细胞 (Tca8113细胞株 )后分别应用3 H TdR掺入法、流式细胞仪 (FCM )、TUNEL法和透射电镜等方法进行检测。结果 :与对照组比较 ,CD/5 FC治疗组3 H TdR掺入率显著下降 (P <0 .0 0 1) ,且与 5 FC的浓度成反比 ;FCM显示S期比率显著升高 (P <0 .0 0 1) ,G2 +M期比率下降为 0 (P <0 .0 0 1) ,G1/G0 期的改变不明显 (P >0 .0 5 ) ;透射电镜及TUNEL法显示治疗后可观察到明显的细胞凋亡 ,其凋亡率由治疗前的 (4 .40± 0 .87) %上升到 (15 .80± 1.5 5 ) % (P <0 .0 0 1)。结论 :AdCMVCD/5 FC系统对口腔鳞癌细胞的杀伤作用可能通过抑制DNA的合成。Objective: To study the therapeutic mechanism of adenovirus mediated cytosine deaminase /5-fluorocytosine (AdCMVCD/ 5-FC) suicide gene system in the treatment of oral squamous carcinoma cells in vitro. Methods: 3H-thymidine ( 3H-TdR) incorporation assay, flow cytometry (FCM), transmission electron microscope and TUNEL (TdT-mediated dUTP Nick End Labeling)assay were used to detected the changes of Tca8113 cells after the treatment with AdCMVCD/5-FC system. Results: After treatment with CD/5-FC system, 3H-TdR incorporation rate of the cells decreased and significantly decreased between different MOI (multiple of infection) at 5-FC 10 -3 mol/L (P<0.001); the cells accumulated in S phase of cell cycle (P<0.001), G 2+M phase dropped to zero (P<0.001) and G 1/G 0 phase didn`t change (P>0.05); many apoptotic cells were found under transmission electron microscope and the positive rate of apoptotic cells increased from (4.40±0.87)% (before treatment) to (15.80±1.55)% (P<0.05) by TUNEL assay. Conclusion: The therapeutic mechanism of AdCMVCD/5-FC suicide gene system on oral squamous cell carcinoma may be the inhibition of DNA synthesis,blockage of cell cycle in S phase and induction of apoptosis.

关 键 词：腺病毒 治疗 口腔鳞癌 CD/5-FC系统 流式细胞仪 FCM 

分 类 号：R739.8[医药卫生—肿瘤]