大鼠局灶性脑缺血后远隔区域病理形态改变  

Pathomorphological changes of remote region in rats after focal cerebral ischemia

作　　者：楚冰[1] 邵国富[1] 

机构地区：[1]苏州大学附属第二医院神经内科,江苏省苏州市215000

出　　处：《中国临床康复》2003年第25期3414-3415,T001,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察大鼠一侧大脑中动脉闭塞(MCAO)后远隔部位(两侧额叶、丘脑、小脑细胞形态学变化)以探讨其发病机制。方法:健康Sprague-Dawley大鼠20只,体质量250～300g,采用线栓法建立大鼠一侧大脑中动脉闭塞模型,按实验需要将实验动物分成正常、单纯缺血两组;用苏木精-伊红染色和末端脱氧核苷酸转移酶介导的d-UTP生物素标记(TUNEL)法观察两侧额叶、丘脑、小脑MCAO后6,24h、1周细胞形态学变化。结果:同侧额叶、丘脑MCAO后6h开始出现TUNEL阳性细胞分别为(34.3±4.0),(42.4±3.0)个,24h后明显增加犤分别为(198.0±2.6),(239.8±4.2)个犦,至1周阳性细胞减少犤分别为(20.0±1.2),(22.2±2.6)个(F=5272.9,5749.4,P<0.05)犦,而对侧丘脑、额叶及两侧小脑未见明显细胞形态学改变。结论:一侧脑梗死后,运动功能的恢复可由脑卒中后远隔部位脑组织功能所促进,提示神经功能联系不能的恢复在脑卒中的康复中起关键作用。AIM:To observe the cell morphology of bilateral frontal lobes, thalami and cer ebellums in rats after unilateral middle cerebral artery occlusion (MCAO) and to investigate its pathogenesis. METHODS:Twenty Sprague-Dawley rats weighing 250-300 g were divided into cont rol group (n=5) and ischemia group (n=15).Unilateral MCAO model was established with thread occlusion. Hematoxylin and eosin (HE) staining and terminal dUTP nic k and labeling (TUNEL) were used to observe the cell morphology at 6, 24 h, 1 d and 1 week after MCAO. RESULTS:TUNEL positive cells in frontal lobe and thalamus of the same hemisphe re were found at 6 h after MCAO(34.3±4.0 and 42.4±3.0 respectively), increas ed significantly at 24 h(198.0±2.6 and 239.8±4.2 respectively), and then dec reased at 1 week(20.0±1.2 and 22.2±2.6 respectively)(F=5 272.9,5 749.4,P< 0.05).There were insignificant changes of cell morphology in contralateral tha lamus, lobe and bilateral cerebellums.

关 键 词：局灶性脑缺血 病理改变 发病机制 细胞形态学 

分 类 号：R743.31[医药卫生—神经病学与精神病学]