脑栓康对D-半乳糖诱导小鼠脑功能衰退的保护作用(英文)  

Protective effects of Naoshuankang against cerebral function deterioration ind uced by D-galactose in mice

作　　者：张爱林[1] 徐秋萍[1] 孙建宁[1] 张静[1] 李朝霞[1] 李云谷[1] 

机构地区：[1]北京中医药大学中药学院药理教研室,北京市100102

出　　处：《中国临床康复》2003年第25期3448-3449,共2页Chinese Journal of Clinical Rehabilitation

基　　金：教育部博士点课题(9933)~~

摘　　要：目的:探讨脑栓康(NSK)对D-半乳糖诱导小鼠脑功能衰退的保护作用。方法:ICR小鼠随机分为空白对照组、模型对照组、银杏叶提取物(天保宁片)40mg/kg组、NSK25mg/kg组、NSK50mg/kg组、NSK100mg/kg组。小鼠每日颈背部皮下注射D-半乳糖120mg/kg,采用水迷宫试验,测试小鼠的学习记忆能力,并通过测定模型小鼠的学习记忆能力和脑组织超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活力、丙二醛(MDA)和脂褐素含量,观察NSK对模型小鼠脑功能衰退的保护作用。结果:NSK25,50,100mg/kg可不同程度地改善模型小鼠的学习能力犤3组小鼠平均到达B点时间为(43±18),(54±29),(59±61)s;而模型对照组到达B点时间为(63±26)s,t=2.525～2.731,P<0.05犦和记忆能力犤NSK25mg/kg组和模型对照组小鼠平均到达B点时间为(25±26),(50±35)s,t=2.254,P<0.05犦。3个剂量均可以增强模型小鼠脑组织SOD,GSH-Px活力,降低MDA和脂褐素含量(t=-2.675～2.712,P<0.05,t=-8.806～15.989,P<0.01)。结论:抑制脑组织脂质过氧化反应,提高脑组织的抗氧化能力,可能是NSK改善行为学异常,防治老年期痴呆的作用机制之一。AIM:To study the protective effects of Naoshuankang (NSK) against the cerebral function deterioration in mice induced by D-galactose. METHODS:A total of 96 ICR mice were randomly divided into blank control group, model group,Taponing group,and 3 NSK groups with varied NSK doses (25, 50 and 10 0 mg/kg respectively).The model of deteriorated cerebral function was induced by daily subcutaneous injection of D-galactose (120 mg/kg) in the mice, and the l earning and memory abilities were assessed by water maze test.The protective eff ects of NSK against the cerebral function deterioration were observed by investi gating the changes in learning and memory abilities, cerebral SOD and GSH-Px ac tivities, and the levels of MDA and lipofuscin. RESULTS:All three NSK doses could improve the learning and memory abilities to different degrees, with the time of the mice with NSK treatment at 25, 50, 100 mg/kg and of those in model groups to arrive at point B being (43±18),(54±29), (59±61),(63±26) s, respectively,(t=2.525-2.731,P< 0.05).For assessment of the memory ability, the time recorded for the mice in NSK(25 mg/kg) and model group s was (25±26),(50±35) s, respectively (t=2.525-2.731,P< 0.05).NSK at the 3 do ses could markedly enhance cerebral SOD and GSH-Px activities, and decrease the levels of MDA and lipofuscin (t=-2.675-2.712,P< 0.05,t=-8.806-15.989,P< 0.0 1).

关 键 词：脑栓康 D-半乳糖 学习记忆能力 脂质过氧化 老年性痴呆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]