238例恶性淋巴瘤患者中性粒细胞碱性磷酸酶染色结果分析  

Analysis of alkaline phosphatase staining results in 238 cases of malignant lymphoma

作　　者：林榕[1] 王时书[1] 周淑芸[1] 徐晓兰[1] 易正山[1] 陈善文 林刚 罗美云[1] 

机构地区：[1]第一军医大学南方医院血液科,广东广州510515 [2]平顶山市舞钢职工医院,河南平顶山462500 [3]总工会金鸡山工人温泉疗养院,福建福州350009

出　　处：《第一军医大学学报》2003年第10期1097-1098,共2页Journal of First Military Medical University

摘　　要：目的了解中性粒细胞碱性磷酸酶(ALP) 染色在淋巴瘤方面的应用是否具有其检测意义。方法将恶性淋巴瘤骨髓未侵犯189例、骨髓侵犯23例及淋巴瘤细胞白血病26例患者的新鲜干燥血片,用偶氮偶联法做ALP染色,将其结果进行分析并与40例正常人的ALP染色结果比较。结果恶性淋巴瘤患者随着病情的进展,其ALP积分逐渐增高,积分>250分的出现率在正常对照、恶性淋巴瘤骨髓未侵犯和骨髓侵犯及淋巴瘤细胞白血病组分别为0%、4.8%、39.1%、53.9%,具有统计学差异。结论随着恶性淋巴瘤的病情进展,其ALP活性可逐渐增高,提示ALP活性的增高,可间接反映恶性淋巴瘤病情的活跃及进展程度,此项化学染色结果对恶性淋巴瘤临床分期及预后的判断具有一定的参考意义。Objective To examine the significance of alkaline phosphatase (ALP) staining in clinical characterization of malignant lymphoma cases. Methods Fresh peripheral blood smear obtained from 238 cases of malignant lymphoma, including 23 cases with bone marrow involvement (BMI) 189 cases without BMI(NBMI), and 26 cases of lymphoma cell leukemia (LCL), were examined by ALP staining, and the results were compared with those of 40 normal control subjects. Results With the gradual progression of lymphoma, the patients tended to have higher ALP scores, and the percentages of the subjects with a score higher than 250 in the control, NBMI, BMI and LCL were 0, 4.8%, 39.1%, 53.9% respectively, showing significant differences between the four groups. Conclusions Increased ALP activity can be indirectly indicative of lymphoma progression, and ALP staining results may provide insight into the clinical staging and prognosis of malignant lymphoma.

关 键 词：恶性淋巴瘤 中性粒细胞 碱性磷酸酶 染色 诊断 

分 类 号：R733.1[医药卫生—肿瘤]