苦豆子生物碱衍生物9002~#急性毒性及体内抑癌活性实验研究  

Experimental Study of Alkanoid Derivative 9002~# on Acute Toxicity and in Vivo Anti - tumor Effects

作　　者：周则卫[1] 刘培勋[1] 向前[1] 李松年[1] 张荷清[1] 王月英[1] 刘正明[1] 徐文清[1] 

机构地区：[1]中国医学科学院

出　　处：《医学研究通讯》2003年第10期30-31,共2页Bulletin of Medical Research

基　　金：天津市自然科学基金(003804511)

摘　　要：目的使用昆明种小白鼠对苦豆子生物碱衍生物9002#进行急性毒性实验.使用IRM-2纯系小鼠进行淋巴瘤和乳腺癌的体内抑癌活性的实验研究.方法急性毒性实验:IP方式给药,测定小鼠LD50.抑癌试验:建立荷瘤小鼠模型,以腹腔给药(ip)方式给予2个不同剂量的药物水溶液,观察瘤重和体重,测定抑瘤率.结果经实验测定9002#的LD50为4362±810mg/kg,毒性较低,经重复试验9002#确有明显的抑癌作用.对淋巴瘤,剂量50mg/kg×10,抑瘤率51.3%～53.5%(P＜0.01),150mg/kg为31.3%～35.7%(P＜0.05);对乳腺癌,剂量50mg/kg×10,抑瘤率为57.1%(P＜0.01),150mg/kg为48.6%(P＜0.01).结论苦豆子生物碱衍生物9002#属低毒有抑癌活性的化合物,对IRM-2纯系小鼠移植肿瘤有明显的抑制作用,以50mg/kg×10剂量组对淋巴瘤和乳腺癌抑制剂果最显著,值得深入研究.Objective Acute toxicity experiment of Sophora alopecuroides alkanoid derivative 9002~# was performed in Kunming mice. In vivo anti - tumor activity tests of 9002~# were performed in inbred strain IRM - 2 mice using lymphoma and mammary adenoma. Methods Acute toxicity experiment: the drug solution was administrated intraperitoneally(ip) , and LD50 was surveyed by conventional method. Anti - tumor tests: tumor- bearing mice models were set up, 2 separate dosages of drug solutions were administrated intraperitoneally(ip), the changes of sarcoma weights and body weights were observed, amd inhibitory rates of tumors were surveyed. Results LD50 of 9002# was determined through the expeer-iment,up to 4362 ±810mg/kg,a less toxicity compound.It was proved by repeated tests that 9002# has a certain tumor inhibitory effect.for lymphoma, 50mg/kg×10 dosage, inhibitory rate was 51.3% -53.5%(P < 0.01), and 150mg/kg×10 dosage that was 31.3% - 35.7%(P< 0.05).for mammary adenoma,50mg/kg×10 dosage,inhibitory rate was 57.1% (P < 0.01) ,and 150mg/kg ×10 dosage that was 48.6%(P< 0.01). Conclusions Alkanoid derivative 9002~# has less toxicity and a certain inhibitory effect to animal implant sarcomas in inbred strain IRM -2 mice.50mg/kg × 10 dosage group has more obvious inhibitory effect to lymphoma and mammary adenoma,it is worthy to study deeply.

关 键 词：苦豆子 生物碱衍生物 9002# 急性毒性 抑癌活性 实验 

分 类 号：R73-36[医药卫生—肿瘤]