肿瘤转移抑制基因的功能定位克隆研究  

Research on Functional Localization and Cloning of Metastasis Suppressor Genes

作　　者：刘虎[1,2] 叶胜龙[1,2] 

机构地区：[1]复旦大学肝癌研究所 [2]复旦大学中山医院,癌变与侵袭原理教育部,重点实验室,上海200032

出　　处：《癌症》2003年第11期1237-1240,共4页Chinese Journal of Cancer

基　　金：国家重点基础研究发展规划项目(973项目)No.G1998051200;国家自然科学基金No.30271459

摘　　要：转移是肿瘤的主要恶性表型,严重影响肿瘤患者治疗的疗效和预后。肿瘤转移抑制基因的发现将为肿瘤转移的诊断和干预治疗提供重要的线索。然而,迄今发现的转移抑制基因还不多,国内尚无相关报道。为了促进转移抑制基因的研究与发现,本文对肿瘤转移抑制基因功能定位克隆的基本原理与技术路线(主要包括微细胞介导的染色体转移,序列标签位点的PCR定位,和自发转移动物实验模型)作一综述,并叙述了通过此技术路线发现转移抑制基因(KAI-1,KiSS-1,MKK4,BRMS1)的经过,以及此技术在前列腺癌、黑素瘤和肝癌转移抑制基因的功能定位克隆中的应用情况。Metastasis is the most lethal attribute of cancer, which severely affects the effectiveness and prognosis of cancer patients. The discovery of metastasis suppressor genes will provide important clues for the predictive diagnosis and interferential therapies of metastasis. However, there have been few metastasis suppressor genes discovered till now. And this kind of research has not been reported domestically yet. In order to promote this research, this paper reviewed the theoretical principles and technical approaches for the functional localization and cloning strategy for metastasis suppressor genes, which mainly include microcell mediated chromosome transfer, PCR analysis of site tagged sites, and spontaneous metastasis analysis. The metastasis suppressor genes, KAI-1, KiSS-1, MKK4, and BRMS1, discovered by this technique and the application of this technique in prostate cancer, melanoma, and liver cancer are also reviewed.

关 键 词：转移抑制基因 功能定位 克隆 预后 肿瘤转移 

分 类 号：R730.2[医药卫生—肿瘤]