舌鳞癌p15和p16蛋白的表达及其意义  

Expression of p15 and p16 Proteins in Tongue Squamous Cell Carcinoma and Their Significances

作　　者：刘习强[1] 曾融生[1] 黄洪章[2] 廖贵清[1] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院 口腔颌面外科,广东广州510055 [2]中山大学附属第二医院口腔颌面外科,广东广州510120

出　　处：《癌症》2003年第11期1214-1218,共5页Chinese Journal of Cancer

摘　　要：背景与目的:多种原发性肿瘤中都存在p15、p16表达异常,但它们与舌肿瘤的关系仍不清楚。本研究旨在探讨二者在舌鳞状细胞癌中的表达特点及其与临床病理和预后的关系。方法:采用免疫组化(超敏SP法)和图像分析技术,检测45例舌鳞癌和10例正常舌组织中p15、p16蛋白表达水平,并将结果与临床病理指标及生存时间进行统计分析。结果:正常舌组织鳞状上皮中均有p15和p16蛋白表达;舌鳞癌p15、p16的缺失率分别为46.7％(21/45)和66.7％(30/45);p16蛋白表达阳性的患者中,80％(12/15)伴有p15蛋白表达,而p15缺失标本中有85.7％(18/21)伴随p16蛋白表达缺失;二者共缺失的患者绝大多数处于临床Ⅲ、Ⅳ期(16/18)。临床Ⅰ、Ⅱ期病例p15和/或p16蛋白表达水平比Ⅲ、Ⅳ期者的表达高(P<0.05);无颈淋巴结转移组蛋白附性表达率高于有转移组(P<0.01)。p15单独或与p16共缺失者与p15和p16蛋白均表达正常者比较,其术后3年生存率降低(P<0.05)。结论:p15和p16蛋白的表达与舌鳞癌的临床分期及颈淋巴结转移有关;p15蛋白单独或与p16共缺失时,舌鳞癌的预后较差。p15和p16蛋白可作为临床评估舌鳞癌浸润、转移和预后的参考指标。BACKGROUND & OBJECTIVE: Abnormal expression of p15 and p16 were commonly found in many kinds of primary tumors, but the possible correlation between p15 and p16 abnormalities and tongue neoplasms is still unknown. This study was designed to investigate the expression of p15 and p16 proteins and their possible correlation with clinicopathology and prognosis of tongue squamous cell carcinoma (TSCC). METHODS: The expression of p15 and p16 were detected by immunohistochemistry (ultra-sensitive SP method) and the results were analyzed quantitatively by imagine cytometry in 45 cases of TSCC and 10 cases of normal tongue tissues, thereafter, the results were analyzed with clinicopathological parameters and survival time. RESULTS: Both p15 and p16 proteins were expressed in squamous epithelia of the normal tongue tissues. The negative expression rates of p15 and p16 protein in TSCC were 46. 7% (21/45) and 66. 7% (30/45), respectively. 80% (12/15) of the p16 positive cases accompanied with p15 positive staining. However, 85.7% (18/21) of the cases with p15 deletion exhibited p16 negative expression. Most of the cases with both p15 and p16 co-deletion were found in stage Ⅲ and Ⅳ (16/18). The expression rates of p15 and/or p16 in stage Ⅰ, Ⅱ were significantly higher than those in stage Ⅲ , Ⅳ( P <0. 05), while p15 and/or p16 expression rates in node-positive groups were much lower than those in node-negative groups ( P <0. 01). p15 deletion, or both p15 and p16 co-deletion group, also had a decreased 3-year survival rate ( P < 0. 05). CONCLUSION: The expression of p15 and p16 protein are closely associated with clinical stages and cervical lymph node metastasis of TSCC. p15 deletion, or both p15 and p16 co-deletion in TSCC can also predict a poor prognosis. p15 and p16 expression can be used as parameters for evaluating clinical stages and metastatic potential as well as prognosis of TSCC.

关 键 词：舌鳞癌 P15蛋白 P16蛋白 预后 检测 

分 类 号：R739.8[医药卫生—肿瘤]