茴三硫对大鼠酒精及四氯化碳致肝纤维化的防治作用  

Effect of anetholtrithione on the prevention and treatment of rat liver fibrosis induced by alcohol and carbon tetrachloride

作　　者：房龙[1] 马安林[1] 李靖涛[1] 王远新[1] 王泰龄[2] 赵洪川[1] 

机构地区：[1]中日友好医院消化内科,北京100029 [2]中日友好医院病理科

出　　处：《胃肠病学和肝病学杂志》2003年第5期429-432,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的　复制大鼠酒精及四氯化碳致肝纤维化动物模型 ,观察茴三硫对其起防治作用的剂量大小。方法　健康雄性Wistar大鼠 60只 ,随机分为 6组 ,实验持续 4周。A组 (空白对照组 ) :生理盐水灌胃 ,每天 1ml/ 10 0g体重。B组 (模型组 ) :白酒灌胃 ,每天 1mg/ 10 0g体重 ,同时CCl4 腹腔内每次注射 0 .0 2 5mg/ 10 0g体重 ,每周 2次。C、D、E组 (茴三硫小、中、大剂量组 ) :在B组基础上预先灌胃茴三硫每天 0 5、1 0、2 .0mg/ 10 0g体重。F组 (阳性对照组 ) :在B组基础上凯西莱预先灌胃 ,每天 10mg/ 10 0g体重。第 2周末、第 4周末分别取血检测ALT、AST、ALP ,GSH Px、MDA等项目。实验结束处死动物 ,取肝组织行病理检验。结果　酒精及四氯化碳可引起大鼠肝功能损害、脂质过氧化加重 ,组织学出现肝纤维化表现。给予茴三硫可明显减轻这些改变。ObjectiveTo reproduce the rat model of li ver fibrosis induced by alcohol and tetrachloride carbon,and observe the protect ive and therapeutic effects of anetholtrithione. MethodsSixty male Wistar rats were divided randomly into six g r oups,and the experiment was continued for 4 weeks.Group A(control group) was per fused gastrically by saline at the dose of 1 ml·100 g -1 ·d -1 .Th e other groups were perfused gastrically by 56% alcohol at the dose of 1 ml·100 g -1 ·d -1 ,and at the same time CCl 4 was injected intraperitone ally at the dose of 0.025 mg/100 g,b.i.w. Group C, D, E(small, middle and large dose of anetholtrithione) was beforehand perfused gastrically by anetholtrithion e at the dose of 0.5, 1.0, 2.0 mg·100 g -1 ·d -1 .Group F (control group of positive drug) was beforehand perfused gastrically by tiopronin at the dose of 10 mg·100 g -1 ·d -1 .At the end of the 2nd week and the 4 th week ALT, AST, ALP, GSH-Px, MDA were examined.When the experiment was over ,rats were euthanized and the histopathologic alterations of liver were observed .ResultsAt the 2nd week and the 4th week, group B were obviously different from group A, C, D, E, F. Group B showed significant heptic injury, i ncluding increased ALT, AST, ALP, MDA and decreased GSH-Px,cellular necrosis, i nflammatory infiltration and liver fibrosis.When compared with group B, the hept ic injury in grouup C, D, E, F were mild, especially group D. ConclusionAnetholtrithione has remarkable prevention and cure action of rat liver fibrosis.

关 键 词：茴三硫 大鼠 酒精 四氯化碳 肝纤维化 肝功能损害 脂质过氧化 

分 类 号：R575.2[医药卫生—消化系统]