AGL患者端粒酶亚单位hTERT、hTEP1的表达  

Expression of telomerase subunit hTERT, hTEP1mRNA in patients with acute granulocyte leukaemia

作　　者：陈凤花[1] 胡丽华[1] 李一荣[1] 王琳[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院输血科,武汉430022 [2]武汉大学生命科学院

出　　处：《临床输血与检验》2003年第4期251-252,共2页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的　检测急性粒细胞性白血病 ( AGL)患者端粒酶亚单位 -人端粒酶逆转录酶 ( h TERT)和端粒酶相关蛋白1 ( h TEP1 )的表达情况 ,并观察不同的亚单位在 AGL发病中的作用。方法　采用 RT-PCR法检测 3 1例 AGL首治患者、2 4例 AGL复发患者、2 1例 AGL完全缓解期患者以及 1 8例健康体检者外周血白细胞端粒酶亚单位 h TERT,h TEP1 m R-NA。结果　 AGL首治患者、AGL复发患者、AGL完全缓解期患者以及健康体检者 h TERT m RNA的阳性率分别为 80 .6%、83 .3 %、9.5 %和 5 .6%。所有标本 h TEP1 m RNA的阳性率为 1 0 0 .0 %。结论　与 h TEP1比较 ,h TERT表达的上调在AGL的发生与复发中具有更重要的作用 ,而且可用于监测治疗是否有效以及治疗后是否复发。Objective To detect the expression of telomerase subunit, human telomerase reverse transcriptase (hTERT) and human telomerase associated protein 1(hTEP1), in patients with acute granulocyte leukaemia(AGL). And to observe the role which different telomerase subunits play during occurrence of AGL.Method RT-PCR was used to detect telomerase subunit hTERT, hTEP1 messenger RNA in 94 peripheral blood white cell samples,concluding 18 healthy individuals,,31 AGL patients at initial presention ,24 AGL at relapse and 21 AGL at complete remission.Results The positive rate of telomerase reverse transcriptase messenger RNA from healthy individuals,AGL at initial presention,AGL at relapse and AGL at complete remission was 80.6%,83.3%,9.5% and 5.6%, respectively. The positive rate of telomerase associated protein 1 in all samples was 100.0%.ConclusionComparing to telomerase associated protein 1,the up-regulation of the expressiom of hTERT plays more important role during occurrence and relapse of AGL,furthermore hTERT may be used to monitor the efficiency of treatment and relapse post treatment.

关 键 词：急性粒细胞性白血病 端粒酶亚单位 HTERT hTEPl RT-PCR法 复发 

分 类 号：R733.71[医药卫生—肿瘤]