转基因人表皮细胞移植猪创面后hEGF表达的实验  

The hEGF expression of gene-transferred human epithelium after its grafting on porcine surgical wound

作　　者：董肇杨 张文奎 于军一 史伟民 田毅华 陈玉林[2] 戴方平[2] 方之扬[2] Yeurgen Kopp 

机构地区：[1]武警总队医院烧伤整形科,上海201103 [2]第二军医大学长海医院烧伤科 [3]德国弗莱堡大学医学院整形科

出　　处：《中华烧伤杂志》2001年第1期22-24,共3页Chinese Journal of Burns

基　　金：国家自然科学基金资助项目!(39580023)

摘　　要：目的 检测转基因细胞移植于猪的创面后有无人表皮细胞生长因子（hEGF）表达。 方法 将种植于生物硅膜上的转基因细胞和正常表皮细胞移植于猪的创面，在移植后第1、3、5、8、12天取移植创面组织活检，采用免疫组织化学方法，测定移植后创面有无hEGF表达。 结果 移植转基因表皮细胞的创面，hEGF表达阳性；移植正常表皮细胞的创面，hEGF表达阴性。 结论 转染hEGF质粒的表皮细胞可在创面表达hEGF。Objective To investigate the hEGF expression of gene-transferred human epithelium after its grafting on porcine surgical wound. Methods Gene-transferred and normal epithelia were implanted on the biological silicone membrane and then grafted on porcine surgical wound.The tissue samples were harvested from the wound and detected for the hEGF expression with immunohistochemical method on 1,3,5,8 and 12 post-grafting days. Results There existed positive hEGF expression in the wound grafted with gene-transferred epithelia and negative hEGF expression in the wound grafted with normal epithelium. Conclusion Gene-transferred epithelium could express hEGF on the grafted wound.

关 键 词：人表皮细胞移植 猪 创面 HEGF 实验 人表皮细胞生长因子 检测 转基因细胞 

分 类 号：R364[医药卫生—病理学]