以龈沟液中性粒细胞弹力酶最高活性率作为定量检测牙周状况的指标(英文)  

Maximal rate of granulocyte elastase activity in gingival crevicular fluid as a quantitative measurement of periodontal conditions

作　　者：金力坚[1] 梁惠强[1] E.F.Corbet L.P.Samaranayake W.I.R.Davies P..Sder 

机构地区：[1]香港大学牙医学院 [2]瑞典卡罗琳斯卡学院

出　　处：《北京大学学报（医学版）》2002年第2期122-132,共11页Journal of Peking University:Health Sciences

基　　金：ThisstudywassupportedbytheUniversityofHongKong (CRCGgrants 337 2 5 4 0 0 0 3) ;HongKongResearchGrantCouncil(RGCgrants ;HKU 72 87 97M)andtheKarolinskaInstitute

摘　　要：目的 :研究人类龈沟液中性粒细胞弹力酶的动态活动方式和最高活性率 ,从而探讨其作为一项定量检测牙周状况指标的可能性。方法 :利用滤纸条法 ,从 33例慢性牙周炎患者提取 2 17份龈沟液样本 ,用中性粒细胞弹力酶的特异性底物对该酶在龈沟液中的活性进行动态定量测量 ,从而计算该酶最高活性率 (MR EA ,单位为每个牙部位mAbs·min- 1 ) ,并将结果与临床牙周状况进行综合比较分析。结果 :龈沟液中性粒细胞弹力酶可分为 5种不同的酶活动方式 (A ,B ,C ,D ,E) ,其相应的MR EA存在显著差异 (P <0 0 1)。在临床牙周状况相同的牙部位 ,弹力酶活动差异有显著性 ,各种临床部位的弹力酶活动方式高 (A % +B % ) 低 (D % +E % )分布情况如下 :健康部位0 % 92 % ,牙龈炎部位 6 % 72 %和牙周炎部位 2 1% 5 4%。健康部位的MR EA值≤ 3 0 ,而病变部位的MR EA值存在明显差异 ,具体分布如下 :75 %牙龈炎部位的MR EA≤ 3 0 ,13% >6 0 ,4% >10 0 ;而 6 0 %牙周炎部位的MR EA值≤ 3 0 ,30 % >6 0 ,2 1% >10 0。结论 :MR EA能作为定量测定龈沟液中性粒细胞弹力酶活动的适当指标 ,可以用来定量检测牙周状况。Objective: To investigate the dynamic pattern of granulocyte specific elastase activity in gingival crevicular fluid (GCF) from subjects with chronic periodontitis, and to evaluate whether this assay could serve for assessment of periodontal conditions. Methods: GCF was collected by paper strips at 217 sites from 33 subjects with untreated chronic periodontitis. Dynamics of the elastase activities in GCF were analyzed with a substrate specific for granulocyte elastase, L pyroglutamyl L prolyl L valine p nitroanilide. Results: Five time dependent, dynamic patterns of elastase activity were defined and named Patterns A to E. The highest slope portion of a plotted substrate hydrolysis time course within a 5 h period was used to calculate the maximal rate of elastase activity (MR EA, mAbs/min/site), while the maximal level of elastase activity (M EA, Abs/site) within that period was also calculated for comparison. Marked differences were found in MR EA among the five patterns ( P <0.01), while measuring M EA alone failed to distinguish between Patterns A and B. Among these sites with high GCF volume and those within varying categories of probing depths with or without bleeding on probing, a large variation in elastase activity was observed. High/low activity patterns, i.e., A & B/D & E, were found respectively in 0%/92% of the healthy sites, 6%/72% of the gingivitis sites, and 21%/54% of the periodontitis sites. A wide range of MR EA values was found among the diseased sites when a maximal MR EA value for healthy sites was delineated at 3.0. Of the gingivitis sites, 75% had values ≤3.0,13%>6.0,and 4%>10.0. The corresponding values for periodontitis sites were 60%, 30% and 21%, respectively. Conclusion: MR EA seems to be an appropriate measurement parameter for assay of GCF granulocyte elastase activity. MR EA may thus serve as a useful host marker for quantitative assessment of periodontal conditions.

关 键 词：龈沟液 中性粒细胞弹力酶 定量检测 牙周状况 牙周炎 最高活性率 

分 类 号：R781.42[医药卫生—口腔医学]