单克隆抗体34βE12染色在前列腺癌鉴别诊断中的价值(附103例报告)  

Clinical value of 34βE12 immunostaining for the differential dianosis of prostatic carcinoma

作　　者：沈丹华[1] 钟大军[1] 虞有智[1] 鲍冬梅[1] 回允中[1] 

机构地区：[1]北京大学人民医院病理科,100044

出　　处：《中华泌尿外科杂志》2003年第4期273-275,共3页Chinese Journal of Urology

摘　　要：目的　探讨前列腺穿刺标本的临床及病理学特征 ,评价细胞角蛋白 34βE12免疫组化染色在诊断中的价值。　方法　复习 10 3例前列腺穿刺标本的临床资料和血清PSA水平以及HE切片 ,采用 34βE12单克隆抗体进行免疫组织化学染色。 　结果　 10 3例中诊断良性前列腺增生 36例 ,低度前列腺上皮内肿瘤形成 (PINⅠ ) 9例 ,前列腺癌 34例 ,高度前列腺上皮内肿瘤形成不能除外癌变 (PINⅡ～Ⅲ ) 2 4例。良性前列腺增生者 34βE12均呈强阳性反应 ;32例前列腺癌中的肿瘤性腺体对 34βE12呈阴性反应 ;2 4例高度PIN疑有癌变者中 ,34βE12呈阴性表达 14例 ,可以诊断为前列腺癌 ,其余 10例34βE12在腺体基底层呈不连续的阳性表达。 30例前列腺癌患者血清PSA平均值为 5 1.3ng ml,其中2 5例PSA >10 .0ng ml,15例PSA >5 0 .0ng ml。　结论　前列腺穿刺标本的病理学诊断应以腺体结构及细胞学特征为依据 ,34βE12阴性表达以及PSA水平持续增高是前列腺癌诊断的重要指标。ObjectiveTo investigate the clinicopathological features of the needle biopsies of prostatic lesions and to evaluate the value of 34βE12 immunostaining for the diagnosis of prostatic carcinoma.MethodsThe clinical data,levels of serum PSA and HE slides of 103 cases of the needle biopsies of prostatic lesions were reviewed.Immunohistochemical stains for 34βE12 were performed.ResultsThe morphological features revealed 36 cases of benign prostatic hyperplasia(BPH),9 cases of low grade prostastic intraepithelial neoplasia (PIN I)and 34 cases of prostatic carcinoma(PC),24 cases of high grade PIN(PINⅡ and Ⅲ) have been suspected of carcinoma.With immunohistochemical stain,all cases of BPH were strongly reactive for 34βE12 while 32 cases of prostatic carcinoma were negative;14 of 24 cases of high grade PIN were negative for 34βE12 and could be diagnosed as PC.The rest 10 of high grade PIN were interruptedly positive in the basal layer for 34βE12.In the 30 cases of prostastic carcinoma,serum PSA levels of 25 cases was over 10 ng/ml and over 50 ng/ml in 15.ConclusionsThe pathologic diagnosis should based on gland architectures and cytologic features.34βE12 negative expression and elevation of serum PSA are also the important criteria for the diagnosis of prostatic carcinoma.

关 键 词：单克隆抗体 34βEl2染色 前列腺癌 鉴别诊断 免疫学技术 

分 类 号：R737.25[医药卫生—肿瘤]