PIP反义核酸对乙肝病毒PRE转录后调节的影响  

The function of antisense PIP RNA on HBV PRE-dependent post-transcriptional regulation

作　　者：邓庆丽[1] 吴燕峰[1] 苏红[1] 段朝晖[1] 邵静[1] 黄志明[1] 罗超权[2] 

机构地区：[1]中山大学孙逸仙纪念医院医学研究中心,广州510120 [2]中山大学中山医学院生化教研室,广州510080

出　　处：《中华微生物学和免疫学杂志》2003年第9期682-685,共4页Chinese Journal of Microbiology and Immunology

基　　金：广东省自然科学基金资助项目 (0 10 72 5 )

摘　　要：目的　研究乙肝病毒转录后调节元件 (PRE)结合蛋白PIP在PRE转录后调节机制中的作用。方法　用质粒pcDNA3.1(- )构建PIP反义核酸真核表达重组体pAS PIP ;用pAS PIP转染HepG2 细胞 ,再将依赖于PRE转录后调节的CAT报告质粒转染上述细胞 ,通过ELISA方法检测CAT的表达水平。结果　成功构建PIP反义核酸真核表达重组体pAS PIP ;基因转染结果显示 ,与转染空载体组相比 ,转染pAS PIP反义核酸组CAT水平下降 2 1.97%。结论　PIP反义核酸可有效地降低依赖于PRE的CAT的表达 ,PIP在PRE转录后调节中起着重要的作用。Objective To study the function of HBV PRE-interacting protein (PIP) on PRE-dependent post-transcriptional regulation. Methods pAS-PIP expressing PIP antisense RNA was constructed with the plasmid pcDNA3.1(-). HepG_2 cells were transfected with pAS-PIP for down-regulating the expression of PIP gene and then transfeced with the CAT reporter plasmid, whose expression was dependent on PRE posttranscriptional regulation. The expression of CAT gene was measured by ELISA. Results pAS-PIP expressing PIP antisense RNA was constructed and the level of CAT in pAS-PIP transfected HepG_2 cells decreased by 21.97% when compared with that in pcDNA3.1(-) transfected HepG_2 cells. Conclusion The level of CAT, whose expression was dependent on PRE function, decreased obviously by PIP antisense RNA. So PIP may play an important role in PRE posttranscriptional regulation.

关 键 词：PIP反义核酸 乙肝病毒 PRE 转录后调节 影响 

分 类 号：R781.5[医药卫生—口腔医学]