纳米级氧化铁-蝎毒素肽靶向神经胶质瘤的实验研究  

Experimental study of using nanometer ferro oxide-CTX to target glioma

作　　者：张诚[1] 钦华[1] 王治国[1] 汪华侨[2] 

机构地区：[1]广州医学院荔湾医院骨科,广州市510170 [2]中山大学中山医学院脑研究室

出　　处：《中华显微外科杂志》2003年第4期276-278,共3页Chinese Journal of Microsurgery

基　　金："十五"国家高技术研究发展计划项目 ( 86 3计划 )( 2 0 0 1AA2 1 80 31 )

摘　　要：目的　探讨纳米级氧化铁蝎毒素肽靶向神经胶质瘤 ,在交变磁场作用下 ,对神经胶质瘤KM小鼠动物模型的治疗效果。　方法　 40只 18～ 2 2g的神经胶质瘤KM小鼠随机均分成 4组。A组 :生理盐水 0 2ml/只 (对照组 ) ;B组 :1‰纳米级氧化铁 15mg/kg ;C组 :单纯氯毒素 (CTX)蝎毒素肽 5万U/支 ;D组 :1‰纳米级氧化铁 CTX微球 15mg/kg。上述药物均为静脉注射 ,每天 1次 ,各组在相同交变磁场参数作用下 ,一个疗程 (6d)后 ,进行KM小鼠右前腋皮下种植的神经胶质瘤的瘤体重量的测定。　结果　A、B、C组和D组瘤重 (g)分别为 2 53± 0 78、2 0 9± 0 96、0 73± 0 18和 0 64± 0 88,B组与A组比较 ,差异无显著性 (P >0 0 5) ;C组与A组、D组与A组比较差异有显著性 (P <0 0 1或 0 0 5) ;B、C组和D组抑瘤率分别为 2 9 6%、46 9%和 79 9% ,D组治疗效果明显优于B组和C组。　结论　初步实验结果显示 ,纳米级四氧化三铁 CTX微球有明显的特异性靶向神经胶质瘤的特性。在交变磁场作用下 。Objective To explore nanometer ferro oxide CTX that targets to glioma, and its therapeutic effiecacy that treats to glioma model in Kunming(KM) mice with alternating magnetic field Methods Forty KM mice which weight was 18～22g were randomly divided into four groups, each group has ten A group: only intravenous injects NS(0 2 ml, control group); B group: intravenous injects 1‰ nanometer ferro oxide(15 mg/kg); C group: intravenous injects CTX(50 000U); D group: intravenous injects 1‰ nanometer ferro oxide CTX(15 mg/kg)(the microsphere which joints with nanometer Fe 3O 4 and CTX) After one course of treatment(six days), measure the weight of the body of glioma which was implanted sustained in right front axillary of the KM mice Result The weight of glioma(g) in A, B, C and D group was 2 53±0 78,2 09±0 96,0 73±0 18 and 0 64±0 88 respectively No significant differences were found between A group and B group( P >0 05); C group compares to B group, P <0 05; D group compares to A group, P <0 01 The rate of inhibitory tumor was 29 6%,46 9% and 79 0% respectively, D group's therapeutic efficacy was significant better than that of B group and C group Conclusion Primary experimental result shows that the microsphere of nanometer Fe 3O 4 CTX has significant specific character which targets to glioma, and has good therapeutic efficacy to treat the glioma model in KM mice with alternating magnetic field

关 键 词：纳米级氧化铁 蝎毒素肽靶向 神经胶质瘤 实验研究 靶向治疗 

分 类 号：R73-36[医药卫生—肿瘤]