Mpl受体介导的HEL细胞电子传递反应的变化  

Mpl Mediated Electron Transfer Response of HEL Cells

作　　者：张晓泉[1] 冯军[2] 郑蕊[1] 伏爽[1] 李莉[1] 慈云祥[2] 王德炳[1] 

机构地区：[1]北京医科大学人民医院,血液病研究所,北京100044 [2]北京大学化学系,北京100871

出　　处：《中国实验血液学杂志》1999年第3期208-212,共5页Journal of Experimental Hematology

基　　金：To whom correspondence should be addressed

摘　　要：为研究Mpl受体介导的HEL细胞电子传递反应的变化，建立了用细胞电极测定HEL细胞电子传递的电化学方法。结果发现，当TPO-Mpl作用后数分钟可以观察到一个向上的“∧”形氯化电位，TPO-Mpl的量大作用出现在5分钟内，TPO-Mpl的解离在二者作用后60分钟趋向明显，240分钟后完全解离。Scatchard分析说明HEL细胞表面有两种Mpl受体亲和力(K1=18pmol/L，K2=1．1nmol／L)。结果提示，TPO与Mpl受体结合迅速，解离缓慢，HEL细胞上存在两种亲和力的Mpl受体。该方法灵敏、方便，可以连续观察，需要细胞少(1．0×10^4细胞)。The Mpl-mediated electron transfer response of HEL cells was studied by the cell oxidation on an pyroly-tic graphite electrode. The cellular electron transfer response during TPO-Mpl interaction occurs in the length of minutes, and an ' A ' shaped upright response was observed. The maximal effect of TPO-Mpl could be achieved within 5 minutes. Dissociation of TPO-Mpl became obvious at 60 minutes and completed 240 minutes after interaction. Scatchard analysis revealed two kinds of Mpl affinity on HEL cells (K1 = 1.8 pmol/L and K2 = 1.11 nmol/L). The method used was sensitive, continuous and easier, and fewer cells (about 1.0 × 104 cells) were needed.

关 键 词：TPO MPL 电子传递反应 受体亲和力 

分 类 号：R733.73[医药卫生—肿瘤]