对中国农业大学鸡资源群进行基因组扫描的初步分析  

Preliminary Analysis on China Agricultural University Chicken Resource Population Based on Genomic Scanning

作　　者：胡晓湘[1] 黄银花[1] 高宇[1] 杜志强[2] 冯继东[1] 徐慰倬[1] 邓学梅[2] 张莹[1] 樊宝良[3] 赵志辉[1] 刘兆良[1] 杨宁[2] 金宁一[4] 吴常信[2] 李宁[1] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100094 [2]中国农业大学动物科技学院,北京100094 [3]河北邯郸农业高等专科学校,邯郸057150 [4]解放军军需大学军事兽医研究所,长春130062

出　　处：《Acta Genetica Sinica》2003年第12期1101-1106,共6页

基　　金：国家重点基础研究发展规划项目 (G2 0 0 0 0 1610 2 )资助~~

摘　　要：利用PCR结合荧光半自动检测技术对中国农业大学鸡资源家系的 1个包括 4 4 0个个体的 3代群体进行了大规模基因组扫描 ,分析了 5 5个微卫星标记位点。经家系分析表明 ,这些位点都符合孟德尔共显性遗传规律 ,其杂合度在 0～ 0 86之间 ,其中 72 %的位点的杂合度 >0 6 0 ;多态信息含量在 0～ 0 88之间 ,73%的位点的PIC >0 5 0。A系和C系群体在 5 5个位点中的绝大多数位点上存在非共有等位基因 ,两个品系在大多数位点的等位基因和等位基因频率差异显著 (P <0 0 5 ) ,许多位点差异达到极显著水平 (P <0 0 1) ;C系显示出更高的群体整齐度。利用 5 5个微卫星位点的等位基因频率 ,计算出A系和C系的奈氏群体间相似系数I和标准遗传距离D分别为0 10 0 2和 0 892 8。Combining the technique of multiplex PCR and the fluorescent semi automated detection,a large scale genome scanning was performed for 440 chickens,which was derived from China Agricultural University chicken resource families,within three generations.Fifty five microsatellite markers were analyzed for this study.Those 55 microsatellite loci accorded with the characters of Mendelian co inheritance.The heterozygosities ranged from zero to 0 89,with 72% of loci having a heterozygosity of more than 0 60.The polymorphism information content ( PIC ) ranged from 0 to 0 85,in which 70% of those loci had a PIC of more than 0 50 but their distribution varied in line A and line C.The allele frequency was significantly different between line A and line C at most loci ( P <0 01).At the same time,gene accordance inclination was found in line C.The Nei population resemble coefficient and standard genetic distance were 0 1002 and 0 8928.

关 键 词：微卫星标记 鸡 基因组扫描 多重PCR 

分 类 号：S831[农业科学—畜牧学]