脱石蜡标本流式细胞DNA分析术对肾细胞癌生物学特性的研究  

THE BIOLOGIC CHARACTERISTICS OF RENAL CELI CANCER STUDIED BY FLOW CYTOMETRIC DNA ANALYSIS OF DEPARAFFINIZED SPECIMENS

作　　者：缪廷杰[1] 王泽[1] 

机构地区：[1]上海医科大学附属中山医院泌尿外科

出　　处：《上海医学》1992年第3期125-128,共4页Shanghai Medical Journal

摘　　要：用流式细胞分析术检测了70例肾细胞癌脱石蜡核悬液标本。结果显示肿瘤细胞异倍体的出现率为58.6％;其中双异倍体的出现率为8.6％。T_1至T_4期肾细胞癌的异倍体出现率依次为13.3％,61.1％,87.5％和100％。S期细胞分数(SPF)在T_1期者为7.4±2.8％,而T_4期者高达23.1±5.4％。上述结果表明随着肾细胞癌病理分期的增高异倍体出现率和SPF明显上升。另外,对16例肾细胞癌的原发癌组织和转移癌组织标本同时作了回顾性流式细胞DNA分析,发现3例(18.75％)肾细胞癌其原发癌组织和转移癌组织DNA倍体类型完全不同。此外,对肾细胞癌瘤块大小和组织学分型与DNA倍体类型和SPF之间的关系作了探讨。Paraffin-embedded tumor specimens from 70 patients with renal cell cancer were studied by DNA flow cytometry. The results showed that aneuploid and two-aneuploid incidences were 58.6% and 8.6% respectively. Aneuploidy was detected in 13.3% of T_1 tumors, 61.1% of T_2 tumors, 87.5% of T_3 tumors and 100% of T_4 tumors. S-phase fraction (SPF) was 7.4+2.8% in T_1 tumors, but 23.1+5.4% in T_4 tumors. The stage of tumor rose with the incidence of aneuploidy and SPF. The metastatic tumor specimens from 16 patients were also studied with DNA flow cytometry and it was found that the DNA ploid patterns were different in 3 cases (18.8%).The relationship between DNA ploid pattern or SPF and tumor size or histological type is discussed.

关 键 词：肾细胞瘤 DNA倍体类型 DNA指数 

分 类 号：R737.11[医药卫生—肿瘤]