放线菌15个属中线型染色体和线型质粒的检测  

Detection of Linear Chromosomes and Plasmids Among 15 Genera in the Actinomycetales

作　　者：马宁[1] 马伟[1] 姜成林[2] 方萍[3] 覃重军[1] 张亚协[3] 

机构地区：[1]中国科学院上海植物生理生态研究所,上海,200032 [2]云南大学微生物研究所,云南,650091 [3]同济大学环境科学与工程学院,污染控制与资源化国家重点实验室,上海,200092

出　　处：《微生物学报》2003年第5期666-670,共5页Acta Microbiologica Sinica

基　　金：上海市自然科学基金 (0 2ZA14 0 96)~~

摘　　要：Bacterial chromosomes and plasmids are commonly circular, however, lin ear chromosomes and plasmids were discovered among 5 genera of the Actinomycet ales. Here, we use pulsed field gel electrophoresis to study the genomes of 19 spe cies which belong to 15 genera in the Actinomycetales. All chromosomes of 19 spe cies are linear DNA, and linear plasmids with different sizes and copy numbers a re detected among 5 species. This work provide basis for investigating the possi ble novel functions of linear replicons beyond Streptomyces and also helps t o develop Actinomycetales artificial linear chromosome.Bacterial chromosomes and plasmids are commonly circular, however, lin ear chromosomes and plasmids were discovered among 5 genera of the Actinomycet ales. Here, we use pulsed field gel electrophoresis to study the genomes of 19 spe cies which belong to 15 genera in the Actinomycetales. All chromosomes of 19 spe cies are linear DNA, and linear plasmids with different sizes and copy numbers a re detected among 5 species. This work provide basis for investigating the possi ble novel functions of linear replicons beyond Streptomyces and also helps t o develop Actinomycetales artificial linear chromosome.

关 键 词：放线菌 线型染色体 线型质粒 检测 

分 类 号：Q939.130[生物学—微生物学]