酵母菌中表达的新疆家蚕抗菌肽(Cecropin-XJ)的特性研究  

Studies on the Properties of Cecropin-XJ Expressed in Yeast from Xinjiang Silkworm

作　　者：刘忠渊[1] 张富春[1] 蔡伦[1] 赵干[1] 王宾[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《微生物学报》2003年第5期635-641,共7页Acta Microbiologica Sinica

基　　金：国家科技攻关<新疆特殊环境生物资源的研究与开发利用>(2 0 0 1BA90 1A3 2 )~~

摘　　要：研究酵母菌中表达的新疆家蚕抗菌肽 (Cecropin XJ)的抗菌特性。根据琼脂孔穴扩散法 ,检测抗菌肽的热稳定性、抑菌效价、对氨苄青霉素抗性菌的抑菌作用 ,并检测了抗菌肽对酸碱盐、人造胃液的耐受性及其抗菌谱。结果显示新疆家蚕抗菌肽具有很强的热稳定性、能够杀灭氨苄青霉素抗性菌、对酸碱盐、人造胃酸有一定的耐受性 ,其杀菌活力为 :1mg抗菌肽与 1 2 0 0U的氨苄青霉素杀菌活力相当。新疆家蚕抗菌肽能够不同程度地杀灭革兰氏阳性菌和革兰氏阴性菌 ,这一发现将为抗菌肽在农业、医疗卫生、畜牧业等方面的应用奠定基础 。The purpose of this study is to investigate the properties of recombinant Cecrop in-XJ isolated from Xinjing silkworm and expressed in Pichia yeast. Accordi ng to Agarose Diffusion Assay, this recombinant Cecropin-XJ has exhibited an ex treme heat-stable characteristic and the ability to kill ampicillin-resistant S. aureus and S. enterica. Moreover, we have observed that the Cecropin -XJ well tolerant to extreme acidic, basic, and high salt environments as well as resistant to 24 hours digestion by artificial gastric juice. The inhibition c apability to S. aureus with 1mg Cecropin-XJ is equal to 1200U ampicillin. W ith a broad spectrum of antibacterial activities, the Cecropin-XJ is able to in hibit the Gram-positive bacteria and Gram-negative bacteria. These findings co uld lead it to a broad applications for agriculture, medical, domestic animal an d food industry. The further investigation of the antibacterial mechanism of cec ropin-XJ is needed.

关 键 词：酵母菌 表达 新疆家蚕抗菌肽 抗菌活性 热稳定性 抑菌效价 人造胃液 抗菌谱 

分 类 号：Q78[生物学—分子生物学]