人降钙素基因相关肽转基因马铃薯的RT-PCR分析  

RT-PCR Analysis of Human Calcitonin Gene-related Peptide Gene Expression in Transgenic Potato

作　　者：宋东光[1] 张英慧[1] 杨泉女[1] 黄碧琳[1] 胡祎娜[1] 聂呈荣[1] 钟希琼[1] 董华强[1] 汪训明[2] 

机构地区：[1]佛山科学技术学院园艺系,广东佛山528231 [2]复旦大学遗传所,上海200433

出　　处：《中国生物工程杂志》2003年第9期96-99,共4页China Biotechnology

基　　金：广东省自然科学基金项目 (No 0 0 0 0 14 )

摘　　要：报道经过农杆菌介导将人降钙素基因相关肽 (calcitoningene relatedpeptide ,CGRP)基因由马铃薯块茎专一表达classIpatatin基因 5′侧翼区和CaMV 35S启动子驱动构建的马铃薯表达载体导入马铃薯 ,PCR鉴定获得了转基因植株。RT PCR分析证实classIpatatin基因 5′侧翼区驱动的CGRPmRNA在转基因马铃薯中的表达。研究结果在开发转基因马铃薯生物反应器表达医用多肽中具有重要意义。flanking region of potato tuber-specific class I patatin gene and CaMV 35S promoter directed human calcitonin gene-related peptide (CGRP) gene expression vectors were transferred into potato by Agrobacterium tumefaciens mediated transformation,as confirmed by PCR.CGRP mRNA expression was further detected by RT-PCR in 5′ flanking region of class I patatin gene directed expression vector transformed plants,this will lay the foundation for producing medicinal peptides in potato bioreactor.;

关 键 词：人降钙素基因 转基因马铃薯 RT-PCR分析 农杆菌介导 

分 类 号：S532[农业科学—作物学] Q943.2[生物学—植物学]