乙烯受体基因LeETR1和LeETR4的克隆及在番茄果实中的表达  

Cloning of Ethylene Receptor Genes LeETR1 and LeETR4 and Their Expression in Tomato Fruit

作　　者：魏绍冲[1] 朱本忠[1] 罗云波[1] 蔚变云[1] 

机构地区：[1]中国农业大学食品学院,北京100094

出　　处：《农业生物技术学报》2003年第2期127-130,共4页Journal of Agricultural Biotechnology

基　　金：国家杰出青年基金(39825118);国家重点基础研究发展规划项目(G1999011701)资助。

摘　　要：为研究野生型番茄(Lycopersiconesculentum Mill.cv.Lichun)和转反义ACS番茄果实中乙烯受体基因LeETR1和LeETR4的表达与乙烯的关系,实验用RT-PCR方法扩增了乙烯受体基因LeETR1和LeETR4片段,用两片段作探针进行Northern杂交。结果表明:两受体基因的表达在番茄果实成熟进程中变化不明显,LeETR4在同一时期的果实外果皮中表达水平低于其在辐射壁和中柱的表达。转反义ACS番茄果实中LeETR1和LeETR4的表达水平显著低于野生型番茄果实,外源乙烯处理转反义ACS番茄果实,促进两个受体基因的表达。可见果实中LeETR1和LeETR4的表达受乙烯调控的影响。To explore the relationship between ethylene and ethylene receptor genes in different developmental stages of wild type tomato(Lycopersicon esculentum Mill cv. Lichun) fruits, as well as in antisense ACS transgenic tomato fruits, LeETR1 and LeETR4 fragments were amplified by reverse -transcription - polymerase -chain -reaction (RT-PCR)from tomato fruit in the experiment. Northern blot analysis indicated that the expression of two ethylene receptor genes in wild type tomato fruit did not differ significantly during fruit ripening, whereas LeETR4 showed lower expression in outer pericarp compared with radial pericarp and columella at the same stage. The level of either LeETR1 mRNA or LeETR4 mRNA in antisense ACS transgenic tomato fruit was significantly lower than that in wild-type fruit. Exogenously applied ethylene could induce the expression of both LeETR1 and LeETR4. The results demonstrated that the expression of LeETR1 and LeETR4 genes could be regulated by ethylene.

关 键 词：乙烯受体 基因 克隆 番茄果实 表达 

分 类 号：S641.2[农业科学—蔬菜学]