利用光钳技术对细胞骨架的分子力学特性的研究进展  

Progress in Studing the Molecular Kinetic Characteristics of the Cytoskeleton by Optical Tweezers

作　　者：徐春华[1] 袁明[1] 王学臣[1] 

机构地区：[1]中国农业大学生物学院植物生理学与生物化学国家重点实验室,北京100094

出　　处：《农业生物技术学报》2003年第4期417-421,共5页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究规划项目(No.G19990117);国家自然科学基金重大课题子课题(19890380)

摘　　要：光钳是近年来发展起来的一种光学物理技术,由于它可以定量测定微小的力(皮牛顿级)和微小的位移(纳米级),近年来广泛应用于生物学领域。细胞骨架是与细胞内各种运动及细胞迁移密切相关、参与产生各种细胞生理过程所需机械力的细胞组分,对其力学性质的研究具有重要意义。对光钳技术的基本原理和测定方法作了简要的介绍,综述了最近10年间利用光钳技术研究细胞骨架的分子力学特性的研究进展及其发展动向。Optical tweezers are up-to-date physical techniques, which are capable of quantitatively measuring tiny forces in pico-Newton and displacements in the nano-meter scale, and have been broadly applied in biological studies. The cytoskeleton is closely related to cellular movements and important cellular components to produce mechanical forces in various cellular processes. In present paper, the basic principle and experimental methods of optical tweezers technique were introduced and recent study progresses in molecular kinetic characteristics of the cytoskeleton by optical tweezers were reviewed.

关 键 词：光钳技术 细胞骨架 分子力学 微丝 微管 马达蛋白 

分 类 号：Q245[生物学—细胞生物学]